Better wastewater treatment? It's a wrap
Rice's trap-and-zap strategy for antibiotic resistant bugs becomes wrap, trap and zap
A shield of graphene helps particles destroy antibiotic-resistant bacteria and free-floating antibiotic resistance genes in wastewater treatment plants.
Improved bacterial affinity and reactive oxygen species generation enhances antibacterial inactivation in wastewater by graphene oxide-wrapped nanospheres developed by scientists at Rice University and Tongji University, Shanghai. Antibiotic resistance genes (eARG) released by inactivated antibiotic resistant bacteria (ARB) in the vicinity of photocatalytic sites on the spheres facilitates their degradation.
Alvarez Research Group/Rice University
Think of the new strategy developed at Rice University as "wrap, trap and zap."
The labs of Rice environmental scientist Pedro Alvarez and Yalei Zhang, a professor of environmental engineering at Tongji University, Shanghai, introduced microspheres wrapped in graphene oxide in the Elsevier journal Water Research.
Alvarez and his partners in the Rice-based Nanosystems Engineering Research Center for Nanotechnology-Enabled Water Treatment (NEWT) have worked toward quenching antibiotic-resistant "superbugs" since first finding them in wastewater treatment plants in 2013.
"Superbugs are known to breed in wastewater treatment plants and release extracellular antibiotic resistance genes (ARGs) when they are killed as the effluent is disinfected," Alvarez said. "These ARGs are then discharged and may transform indigenous bacteria in the receiving environment, which become resistome reservoirs.
"Our innovation would minimize the discharge of extracellular ARGs, and thus mitigate dissemination of antibiotic resistance from wastewater treatment plants," he said.
The Rice lab showed its spheres -- cores of bismuth, oxygen and carbon wrapped with nitrogen-doped graphene oxide -- inactivated multidrug-resistant Escherichia coli bacteria and degraded plasmid-encoded antibiotic-resistant genes in secondary wastewater effluent.
The graphene-wrapped spheres kill nasties in effluent by producing three times the amount of reactive oxygen species (ROS) as compared to the spheres alone.
The spheres themselves are photocatalysts that produce ROS when exposed to light. Lab tests showed that wrapping the spheres minimized the ability of ROS scavengers to curtail their ability to disinfect the solution.
The researchers said nitrogen-doping the shells increases their ability to capture bacteria, giving the catalytic spheres more time to kill them. The enhanced particles then immediately capture and degrade the resistant genes released by the dead bacteria before they contaminate the effluent.
"Wrapping improved bacterial affinity for the microspheres through enhanced hydrophobic interaction between the bacterial surface and the shell," said co-lead author Pingfeng Yu, a postdoctoral research associate at Rice's Brown School of Engineering. "This mitigated ROS dilution and scavenging by background constituents and facilitated immediate capture and degradation of the released ARGs."
Because the wrapped spheres are large enough to be filtered out of the disinfected effluent, they can be reused, Yu said. Tests showed the photocatalytic activity of the spheres was relatively stable, with no significant decrease in activity after 10 cycles. That was significantly better than the cycle lifetime of the same spheres minus the wrap.
Original publication
Other news from the department science
Most read news
More news from our other portals
Last viewed contents
Evotec and Secarna form strategic partnership in the field of antisense therapy - Comprehensive agreement for the development of novel antisense oligonucleotide therapeutics across a broad range of targets and indications
Implantable device cuts obstructive sleep apnea symptoms - Offers promise for patients unable to tolerate CPAP
Evotec, Inserm, Lille University Hospital and Inserm Transfert enter collaboration - Identification of novel therapeutic targets in obesity and metabolic diseases
Mount Sinai researchers find potential therapeutic target across a range of cancer types
UV Mini-Spectrometer C16767MA | UV spectrometers | Hamamatsu Photonics
CytomX and MD Anderson Cancer Center enter collaboration for Probody-enabled CAR-NK cell therapies
Max-Planck-Institut für Neurobiologie - Martinsried, Germany
Bracco Imaging and Shanghai Pharmaceuticals join forces in the field of ultrasound
Vantia Therapeutics’ VA111913 Enters Phase II Proof-of-Concept Trials for Dysmenorrhoea
Category:Restorative_dentistry
New study sheds light on genetics of rice metabolism
