Trapping and watching motile cells
A new approach enables rapid characterization of living suspension cells in 4 dimensions
Holographic optical tweezers (HOT) are well suited to handle motile cells. And they can greatly increase experimental throughput by trapping an array of cells in a single field of view. Yet, researchers struggle with a particular problem, when they combine optical tweezers with confocal microscopy: an axial change in the position of the focus typically requires a corresponding axial translocation of the objective lens. This, however, affects both the imaging and trapping planes simultaneously, making it impossible to trap a cell and to determine its 3D morphology at the same time.
An international research team from the University of California (Davis, Sacramento, USA), the University of Ulm (Germany), the University of Gothenburg (Sweden), and the University of Bielefeld (Germany) now developed a solution to overcome these limitations. They designed an optical system based on a combination of spinning disk confocal fluorescence microscopy and holographic optical tweezers. They utilize a spatial light modulator (SLM) and a voltage-controlled feedback-system to induce a shift in the trapping plane, equal and opposite to the movement of the objective and image plane. In this way, full control over the position and orientation of multiple trapped cells including directed motion and object rotation are possible, while also allowing rapid 4D imaging.
An immediate benefit from this system is the ability to streamline and multiplex imaging experiments. Multiple objects of interest can be pulled and held in a single FOV, instead of imaging only one cell or interaction at a time. Similarly, multiple interactions may be able to be induced and maintained in a single FOV, such as those between a host and pathogen. Furthermore, trapped objects could be rotated to bring specific cellular regions, such as those with a higher (or lower) local concentration of surface receptors, into contact with target areas.
The researchers demonstrated the capabilities of their new setup by imaging and manipulating living, fluorescently stained Jurkat T cells. Structures of cells immobilized within the optical trap could be resolved in a useful z-range of 0.3–30 µm, which covers all non-adherent mammalian cells.
Original publication
Other news from the department science
Most read news
More news from our other portals
See the theme worlds for related content
Topic world Fluorescence microscopy
Fluorescence microscopy has revolutionized life sciences, biotechnology and pharmaceuticals. With its ability to visualize specific molecules and structures in cells and tissues through fluorescent markers, it offers unique insights at the molecular and cellular level. With its high sensitivity and resolution, fluorescence microscopy facilitates the understanding of complex biological processes and drives innovation in therapy and diagnostics.
Topic world Fluorescence microscopy
Fluorescence microscopy has revolutionized life sciences, biotechnology and pharmaceuticals. With its ability to visualize specific molecules and structures in cells and tissues through fluorescent markers, it offers unique insights at the molecular and cellular level. With its high sensitivity and resolution, fluorescence microscopy facilitates the understanding of complex biological processes and drives innovation in therapy and diagnostics.