Achieving Method Modernization with the New Liquid Chromatographic Gradient Allowances
Waters S.A.S.
The extent to which the various parameters of a chromatographic test may be adjusted without fundamentally modifying the pharmacopeial analytical procedures is defined in U.S. Pharmacopeia (USP) General Chapter <621> Chromatography. In this application brief, we combine the gradient method adjustments described in this chapter with the Alliance™ iS HPLC System to achieve both column dimension and system modernization for the USP monograph separation of antiviral drug, abacavir sulfate.
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Achieving Method Modernization with the New Liquid Chromatographic Gradient Allowances