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Chromatography columns:

Superdex™ 30 Increase, Superdex 75 Increase, Superdex 200 Increase, Superose™ 6 Increase

New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins

Cytiva Europe GmbH

Superdex 75 Increase column use on ÄKTA pure
SEC Increase prepacked column formats
Comparison between Superose 6 Increase and Superdex 200 ...
Comparison between Superose 6 Increase and Superdex 200 ...
Superdex 75 Increase column use on ÄKTA pure
SEC Increase prepacked column formats
Comparison between Superose 6 Increase and Superdex 200 ...
Comparison between Superose 6 Increase and Superdex 200 ...

Maximized Resolution and Optimized Run Time for Small-Scale Preparative Purification and Analysis

The new generation size exclusion chromatography columns called Increase” from GE Healthcare offer improved performance than their predecessors. 

Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity than their predecessors, they offer:

  • Reduced runtimes with maintained resolution for results faster
  • Higher resolution for improved purity
  • Higher lot to lot consistency

The columns are tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over

With the addition of Superdex™ 30 Increase in June 2017, you now have four choices of the new generation SEC resin for small-scale preparative purification and analysis (4 to 500 µl):

  • For peptides and other small biomolecules: Superdex 30 Increase (Fractionation range Mr 100 to 7000)
  • For recombinant tagged proteins: Superdex 75 Increase (Fractionation range Mr 3000 to 70 000)
  • For MAbs and other antibodies: Superdex 200 Increase (Fractionation range Mr 10 000 to 600 000
  • For larger proteins and protein complexes: Superose™ 6 Increase (Fractionation range Mr 5000 to 5 000 000)

Three of the resins (Superdex 75 Increase, Superdex 200 Increase and Supersoe 6 Increase) are available prepacked in high-performance columns of three different sizes:10 mm x 300 mm (24 ml column volume), 5 mm x 150 mm (3 ml column volume) and 3.2 mm x 300 mm (2.4 ml column volume). Superdex 30 Increase is available as 10 mm x 300 mm and 3.2 mm x 300 mm columns. All columns are made of glass to allow easier visual inspection of the packed bed. The columns have fittings for simple connection to ÄKTA™ chromatography systems or equivalent high-performance systems.

Three different column dimensions are available:

  • The 10 × 300 mm column is well suited for high resolution separations for both preparative and analytical purposes, with sample volumes from 25 µl to 500 µl
  • For smaller sample volumes (4–50 µl) and high resolution preparations and analyses, the 3.2 × 300 mm column is the best choice
  • The shorter 5 × 150 mm column is suitable for quick purity checks and rapid screening when the highest resolution is not needed

Check what our customers are saying on the product website.

Superdex 75 Increase column use on ÄKTA pure

1

Superdex 75 Increase column use on ÄKTA pure

SEC Increase prepacked column formats

2

SEC Increase prepacked column formats

Comparison between Superose 6 Increase and Superdex 200 ...

3

Comparison between Superose 6 Increase and Superdex 200 Increase. Superose 6 Increase gives higher resolution for proteins with Mr ≥ 440 000, while proteins of lower molecular weight are better resolved on Superdex 200 Increase

Comparison between Superose 6 Increase and Superdex 200 ...

4

Comparison between Superose 6 Increase and Superdex 200 Increase. Superose 6 Increase gives higher resolution for proteins with Mr ≥ 440 000, while proteins of lower molecular weight are better resolved on Superdex 200 Increase

5

Request information about Superdex™ 30 Increase, Superdex 75 Increase, Superdex 200 Increase, Superose™ 6 Increase now

Superdex 75 Increase column use on ÄKTA pure

Chromatography columns: Superdex™ 30 Increase, Superdex 75 Increase, Superdex 200 Increase, Superose™ 6 Increase

New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins

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Find Superdex™ 30 Increase, Superdex 75 Increase, Superdex 200 Increase, Superose™ 6 Increase and related products in the theme worlds

Topic world HPLC

HPLC is a key technology in modern analytical chemistry. It enables the separation, identification and quantification of components in complex mixtures with high precision and efficiency. Whether in the analysis of active pharmaceutical ingredients, the quality control of foodstuffs or the examination of biological samples - HPLC is often the method of choice for demanding separation tasks.

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View topic world
Topic world HPLC

Topic world HPLC

HPLC is a key technology in modern analytical chemistry. It enables the separation, identification and quantification of components in complex mixtures with high precision and efficiency. Whether in the analysis of active pharmaceutical ingredients, the quality control of foodstuffs or the examination of biological samples - HPLC is often the method of choice for demanding separation tasks.

20+ products
5+ whitepaper
15+ brochures

Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

50+ products
10+ whitepaper
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View topic world
Topic World Chromatography

Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

50+ products
10+ whitepaper
45+ brochures