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Vericheck ddPCR™ Empty-Full Capsid Kit
Empty-to-Full Capsid Ratio Analysis For AAV-based Gene Therapy
Sample Type - Crude lysate or purified samples, from as little as 2 µl (106 virus particles)
Faster, highly precise results - CV <5%, up to 14 samples per plate in less than 1 day
Simplified data analysis –simultaneous results for genome titer, capsid titer, empty-full ratio
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Results within a day! Accurately measure protein & DNA simultaneously using ddPCR Technology
The empty-to-full capsid ratio is a critical quality attribute (CQA) that influences the efficacy and safety of gene therapy treatments. Achieving optimal therapeutic outcomes in gene therapy relies on precise viral vector characterization.
Bio-Rad’s Vericheck ddPCR Empty-Full Capsid Kit uses the unparalleled precision of Droplet Digital PCR (ddPCR) to accurately measure protein and DNA simultaneously in one well. The ability to leverage the same assay for both crude and purified samples and provide results within a day enables expedited answers for the entire adeno-associated virus (AAV) production workflow.
Unlike other available technologies, this kit provides consistent and reliable capsid content measurements across the AAV production process. The Vericheck ddPCR Empty-Full Capsid Kit simplifies your AAV development to achieve reliable results with this groundbreaking innovation. Absolute quantification can be easily and quickly obtained via ddPCR technology for AAV serotypes 2, 5, 8 and 9.
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Method Overview
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2-D amplitude plot for comprehensive ddPCR analysis
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Fig. 3. Low coefficient of variation (AAV9). Fig. 4. Repeatability
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Fig. 5. With ddPCR technology, only ITR-2 is detected, not contaminants, ensuring accurate categorization of full capsids.
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PCR kits: Vericheck ddPCR™ Empty-Full Capsid Kit
Empty-to-Full Capsid Ratio Analysis For AAV-based Gene Therapy
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Find Vericheck ddPCR™ Empty-Full Capsid Kit and related products in the theme worlds
Topic World PCR
This groundbreaking and highly versatile molecular technique of PCR allows us to amplify tiny amounts of genetic material on a large scale and analyze them in detail. Whether in medical diagnostics, forensic DNA analysis or research into genetic diseases - PCR is an indispensable tool that gives us deep insights into the world of DNA. Immerse yourself in the fascinating world of the polymerase chain reaction (PCR)!
Topic World PCR
This groundbreaking and highly versatile molecular technique of PCR allows us to amplify tiny amounts of genetic material on a large scale and analyze them in detail. Whether in medical diagnostics, forensic DNA analysis or research into genetic diseases - PCR is an indispensable tool that gives us deep insights into the world of DNA. Immerse yourself in the fascinating world of the polymerase chain reaction (PCR)!