SkillPak pre-packed columns

Pre-packed chromatography columns for purification are ready-to-use, available with different kinds of chromatography resins. Sizes range from milliliter scale for screening and method development up to tens of liters for GMP manufacturing. The columns offer significant advantages over traditional user-packed columns by eliminating the need for laborious column packing procedures and ensuring consistent and reproducible results.

Benefits of pre-packed columns - Enhancing Bioprocessing Efficiency

Pre-packed columns are designed to maximize process efficiency, providing the following benefits for bioprocessing workflows:

• Time Savings: By eliminating column packing preparation time is significantly reduced, allowing researchers to focus more on their experiments and accelerate project timelines.
• Improved Consistency and Reproducibility: Precise resin packing ensures consistent bed heights and uniform flow distribution, leading to reproducible results and better process control.
• Reduced Risk of Contamination: As the columns are sealed and sterilized, the risk of contamination during packing is eliminated, safeguarding the integrity of valuable samples and minimizing the chance of rework.
• Ease of use: The columns are delivered ready-to-use with all documentation such as HETP and AS for almost all columns and bioburden and endotoxin test for columns used in manufacturing. The following video shows what to expect when ordering pre-packed columns (SkillPak™) and how to connect them to a chromatography system.

Applications of pre-packed columns

Pre-packed chromatography columns can be used at all stages of a (bio)pharmaceutical product: from delevepment to large scale production. Column suppliers support this by offering a wide range of products tailored for different applications.

• Chromatography Resin Screening: Small columns pre-packed with a variety of resins enable rapid and efficient screening of various chromatography resins.
• Method Development & Scale-Up: Columns with increasing volumes facilitate a fast and efficient method development & scale-up. An example is shown in the application note “Development and scale-up of an antibody platform using 1 mL to 200 mL SkillPakTM pre-packed columns”
• (Pre-)Clinical Manufacturing: GMP-ready columns are ideally suited for purifications during (Pre-)clinical manufacturing as these columns are often only used for short campaigns.
• Commercial Manufacturing: With the rising popularity of single-use manufacturing, pre-packed columns streamline the purification process of all biopharmaceuticals.
• Multi-Column & Continuous Chromatography: Due to their very low column-to-column variability, pre-packed columns provide best performance for multi-column & continuous chromatography. An example are Tosoh Bioscience’s SkillPak BIO columns, which are dedicated columns for this application.