Eppendorf Simple and Rapid Isolation of Plant RNA Using the Perfect RNA, Eukaryotic Maxi Kit
04.03.2002
The isolation of plant RNA is a particularly challenging task. Many protocols exist for the isolation of total RNA from plants that utilize solvents such as phenol or chloroform. However, their toxicity and the resulting cumbersome disposal make alternative protocols desirable. This application protocol demonstrates that the Perfect RNA TM Maxi Kit for eukaryotic cells is ideally suited for the isolation of total RNA from plant material, as has been demonstrated here with the sources Arabidopsis thaliana (dicotyledon)and Zea mays (monocotyledon).
The technology of the Perfect RNA TM Kit is based on cell lysis with a chaotropic guanidinium isothiocyanate solution, resulting in the rapid inactivation of cellular RNases. Next, the RNA in the lysate is selectively bound to a specially designed matrix and purified from DNA and other contaminants by efficient washing steps. Finally, RNA is eluted in molecular biology grade RNasefree)water.
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