Nanotechnology reveals hidden depths of bacterial 'machines'
New research from the University of Liverpool, has probed the structure and material properties of protein machines in bacteria, which have the capacity to convert carbon dioxide into sugar through photosynthesis.
This is an illustration of a carboxysome.
Dr Luning Liu, University of Liverpool
Cyanobacteria are a phylum of bacteria that produce oxygen and energy during photosynthesis, similar to green plants. They are among the most abundant organisms in oceans and fresh water. Unique internal 'machines' in cyanobacteria, called carboxysomes, allow the organisms to convert carbon dioxide to sugar and provide impacts on global biomass production and our environment.
Carboxysomes are nanoscale polyhedral structures that are made of several types of proteins and enzymes. So far, little is known about how these 'machines' are constructed and maintain their organisation to perform carbon fixation activity.
Researchers from the University's Institute of Integrative Biology, led by Royal Society University Research Fellow Dr Luning Liu, examined in depth the native structure and mechanical stiffness of carboxysomes using advanced microscopes and biochemical approaches.
For the first time, the researchers were able to biochemically purify active carboxysomes from cyanobacteria and characterize their carbon fixation activity and protein composition. They then used electron microscopy and atomic force microscopy to visualise the morphology and internal protein organization of these bacterial machines.
Furthermore, the intrinsic mechanical properties of the three-dimensional structures were determined for the first time. Though structurally resembling polyhedral viruses, carboxysomes were revealed to be much softer and structurally flexible, which is correlated to their formation dynamics and regulation in bacteria.
Dr Liu, said: "It's exciting that we can make the first 'contact' with these nano-structures and understand how they are self-organised and shaped using state-of-the-art techniques available at the University. Our findings provide new clues about the relationship between the structure and functionality of native carboxysomes."
The self-assembly and modularity features of carboxysomes make them interesting systems for nanoscientists, synthetic biologists and bioengineers, who hope to find ways to design new nanomaterials and nano-bioreactors.
"We're now just starting to understand how these bacterial machines are built and work in nature. Our long-term vision is to harness the knowledge to make further steps towards better design and engineering of bio-inspired machines," added Dr Liu, "The knowledge and techniques can be extended to other biological machines."
Original publication
Other news from the department science
These products might interest you
Biostat STR by Sartorius
Biostat STR Generation 3 Bioreactors
Engineered for Ultimate Upstream Performance
Ambr® 250 Modular by Sartorius
Mini bioreactors for cell and gene therapies with high scalability
Maximise your process development with reliable single-use vessels
Ambr® 250 HT Consumables by Sartorius
Efficient bioprocesses with single-use bioreactors
Minimise cleaning effort and maximise flexibility for cell and microbial cultures
Brooks Instrument SLA Biotech-Serie by Brooks Instrument
Control bioprocesses efficiently and precisely with flow controllers for biotechnology applications
The SLA Biotech series has been developed specifically for the requirements in bioprocesses
Flexcell Systems by Dunn
Flexcell Cell Stretching Bioreactors for Life Science Research
Used in over 1300 laboratories worldwide, and cited in over 4000 research publications
SLAMf Biotech-Serie Massendurchflussregler by Brooks Instrument
SLAMf Mass Flow Controllers Designed Specifically for Biotech (IP66 / NEMA 4X)
Special equipment for biotech process plants, suitable for splash water and high-pressure cleaning
