The tightest non-aminoglycoside ligand for the bacterial ribosomal RNA A-site

17-Oct-2018 - Japan

A research group at Tohoku University has made a significant discovery with positive implications for the development of bacteria-fighting drugs. The aminoacyl-tRNA site (A-site) of the 16S RNA decoding region in the bacterial ribosome looks promising for a new era of antibiotic drug development.

Traditional aminoglycoside antibiotics are problematic given their high toxicity and the potential for resistance development. The research at Tohoku University focused on bacterial A-site binding small ligands whose structures are distinct from the aminoglycoside family, which offer potential for the development of novel drugs that treat bacterial infections with a reduction in the problems associated with traditional antibiotics.

The research group led by Dr. Seiichi Nishizawa and Dr. Yusuke Sato (Department of Chemistry, Graduate School of Science) has reported a novel small ligand, ATMND-C₂-NH₂ that has the tightest binding affinity for the bacterial A-site among the non-aminoglycoside ligands.

ATMND-C₂-NH₂ shows a significant fluorescent quenching response upon selective binding to the internal loop of the bacterial (Escherichia coli) A-site-containing model RNA. ATMND-C₂-NH₂ has also proven useful as an indicator for assessing ligand/A-site interactions.

The results obtained by the research group offer a rational basis for the generation of novel A-site binding ligands with a view toward novel antibiotics with less toxicity and minimum resistance development.

Original publication

Dr. Yusuke Sato, Masafumi Rokugawa, Sho Ito, Sayaka Yajima, Hiroki Sugawara, Dr. Norio Teramae Dr. Seiichi Nishizawa; "Fluorescent Trimethylated Naphthyridine Derivative with an Aminoalkyl Side Chain as the Tightest Non‐aminoglycoside Ligand for the Bacterial A‐site RNA"; Chem. Eur. J; 2018

https://www.bionity.com/en/news/161871/the-tightest-non-aminoglycoside-ligand-for-the-bacterial-ribosomal-rna-a-site.html