Like flocks of birds: How cancer cells develop metastases
"Next -we worked to identify the molecular mechanisms and biological processes that increase the migration potential and resistance in collective aggregations compared to solitary cancer cells," explains Giorgio Scita, director of the Mechanisms of Tumor Cell Migration research unit at IFOM and professor at the University of Milan. Using a combination of real-time microscopy, in vitro chemotaxis assays and advanced imaging techniques, it was possible to monitor the phenomenon ni in real time. "At low chemokine concentrations a solitary malignant B-cell does not migrate, whereas an aggregate of cells shows increased migratory capacity. If the chemotactic stimulus is intensified, the single cell undergoes a reverted migration : the excess "fuel" drives it, but once the lymph node is reached it turns back. In contrast, the aggregated cells continue to move forward," continues Gema Malet-Engra, first author of the study.
Analyzing these migrating cells using physical parameters similar to those used in the field of ethology, the researchers found that the cancer cells have behavioral and relational dynamics identical to those typical of migratory birds or sardines, which tend to move in groups to confuse an attacker. "We observed that, whereas the individual components appear move in a random fashion, in reality they maintain perfect coordination in the way they migrate. There are lead cells that drive the movement. These would be subject to a progressive decrease in efficiency and motility due to frictional forces encountered during migration, like a single cell. But within the group there is constant substitution of the leader cells with follower cells. This continuous rotation (every 8-15 minutes) moves fresh cells that have not been weakened to the outside. A mathematical model developed with colleagues from the Weizmann Institute and analysis software developed with colleagues from Singapore have identified for the first time the vectors representing motility, speed and direction of each individual cell and those that identify their ‘flight strategy': rotation among leaders and followers in response to chemotactic stimuli,” continues Scita.
The parameters used also describe the alternation of phases that characterize the migration process, which are closely interconnected with the concentration of chemokine in the environment that they cross: movement is compact and directional when the concentration is high. Group of cells, however, alternate this running state with pauses during which cells within the group move in a rotational or random fashion when the chemokine is scarce, This pausing allows the group to sample the environment and locate higher concentrations and change course.
"It emerges that there is a flawless cell-cell communication mechanism behind this ability to aggregate and migrate efficiently. Prospectively, the clinical objective will be to identify an inhibitor that interferes with this intercellular communication mechanism, disrupting the group and eliminating or reducing migration and chemotaxi," concludes Scita.