Discovery of “probiotic transporters” unlocks secrets of infection-preventive bifidobacteria
Researchers at RIKEN, Yokohama City University and the University of Tokyo have uncovered how gut bifidobacteria protect the body against lethal infection by enhancing the defenses of colonic epithelium. Published in this week’s issue of Nature, the finding provides first-ever clues on the mechanisms underlying the beneficial effects of gut microbiota, promising more effective probiotic therapies for a variety of disorders and diseases.
Survival rate of germ-free mice, preventive B. longum (BL)- and non-preventive B. adolescentis (BA)-associated mice after O157 infection (n = 10).
Schematic representation of the mechanisms of O157 lethal infection and prevention by bifidobacteria. Both B. longum (BL) and B. adrescentis (BA) possess glucose transporter(s) and can produce acetate from glucose in the proximal colon, where glucose is thought to be present; this acetate exerts an anti-apoptotic effect on colonic epithelium. In the distal colon, in contrast, only fructose is available, and thus only BL, with its fructose transporters, is able to produce acetate there. BA lacks the fructose transporters, and thus fails to produce enough acetate to prevent epithelial apoptosis by O157. This results in translocation of Shiga toxin into the blood stream, leading to death of the mice.
In recent years, new metagenomics techniques have enabled scientists to delve ever-deeper into the world of gut microbiota, revealing the strong influence that intestinal bacteria exert on our health. Bifidobacteria, one of the most numerous such bacteria, confer to their hosts a range of beneficial health effects, aiding in digestion, boosting the immune system and even reducing cancer risk. The mechanism underlying these effects, however, has remained a mystery.
With their study, the research team set out to unravel this mystery using a combination of techniques from genetics, transcriptomics and metabolomics. Initial experiments on so-called germ-free (GF) mice, whose guts are uncolonized by bacteria, revealed stark differences between bifidobacteria strains. The researchers found that mice colonized by one bifidobacterium subspecies, B. longum, were able to survive when fed the pathogenic bacteria E. coli O157, while GF mice without the bacteria died of infection within 7 days. Another strain named B. adolescentis, in contrast, had no such effect.
By analyzing fecal metabolic profiles, the researchers succeeded in pinpointing the source of this difference in the production of acetate, which they showed enhances intestinal epithelial defense and protects against infection from O157. The key actor in this mechanism is a carbohydrate transporter encoded by genes present in certain strains of bifidobacteria such as B. longum, which enables these bacteria to utilize fructose to produce acetate in the distal colon.
As a demonstration of the power of multi “omics” technologies, the identification of these “probiotic transporters” constitutes a milestone in the study of gut microbiota. The finding also demonstrates the power of multi “omics” technologies for analyzing the gut ecosystem, promising advancements in the development of cutting-edge probiotic therapies.
Original publication
Other news from the department science
Most read news
More news from our other portals
Last viewed contents
List_of_biomolecules
Category:Carotenoids
Acetylation: a Time-Keeper of glucocorticoid Sensitivity - Understanding the regulatory mechanism paves the way to enhance the effectiveness of anti-inflammatory therapies
When does it make sense to develop a new therapeutic agent? - Criteria that research must meet so that a substance that shows promise in the laboratory can actually be developed into a clinically effective drug
New drug candidates from genome sequence - As proof-of-principle, the team designs potent anti-cancer compound
Myoglobin
Kidney
A building block for liver fitness in old age - Researchers discover cellular protection against epigenetic changes
A greener and cheaper technique for biofuel production - Bacterium isolated from mushroom converts plant material directly to butanol
Analytik Jena appoints new managing director at Japanese subsidiary
Diaphragmatic_breathing
