Programming with the Light Switch
How to control individual components of self-assembling molecular structures
Michal Rössler
Using biological models such as microtubules the researchers are developing self-assembling structures. Microtubules are protein complexes that form a dynamic scaffolding structure in the cells of plants, animals and humans. Their self-assembling structure means that microtubules are constantly forming and degrading at the same time. This allows the scaffolding to adapt easily to changing situations and to react quickly to stimuli by rearranging the building blocks. These processes are driven by a constant dissipation of energy, i.e. a conversion of energy, which the organism regulates via feedback mechanisms. The structures of autonomously acting materials such as those developed by the scientists in the livMatS cluster of excellence should be similarly adaptable in the future. This can be achieved with systems, in which an energetic activation and deactivation take place causing the structural formation and degradation of building blocks.
In their work, the Freiburg researchers add the energy supplier adenosine triphosphate (ATP) to the DNA building blocks in such a system. The scientists have installed molecular photoswitches on one side of the ATP. These react to light by dropping when specifically irradiated and releasing the ATP as an effective fuel molecule for the system. The control over the photoswitches is influenced by the wavelength of the light, the duration of the irradiation and the light intensity. The specific activation of ATP in turn triggers a process: an enzyme closes a bond that forms longer strands from the DNA monomers. Another enzyme, which can recognize and cut DNA at certain positions, cleaves the binding sites again. This results in a simultaneous formation and degradation of the building blocks. During this process the individual DNA building blocks combine to form a polymer.
“Our long-term goal is to use the biological fuel ATP to develop synthetic materials that at least blur the line between living and dead matter,” explains Andreas Walther. “If we are able to use ATP as fuel and convert chemical energy into work, we can design the next generation of implant materials that can actively change and truly interact with the human body.”