Seeing memories being made
Researchers at Technische Universität Kaiserslautern have developed a novel tool for tracking and manipulating long-term memories as they are stored in the brains of fruit flies. The tool, reported recently in the journal PLOS Biology, will help investigate how memory works at the molecular level. Insights gained from the simpler fruit fly brain can later guide studies of human memory.
The researchers (left to right): Vanessa Bräuler, Benjamin Escribano, Dominique Siegenthaler and Prof. Dr. Jan Pielage
Koziel/TUK
Sorting out the specific mechanisms that lead to long-term memory formation in the fly brain is a challenge. The subset of neurons thought to be responsible for long-term memory have the same DNA as other neurons, making it difficult to design a biomarker that only identifies the neurons encoding a specific memory.
To overcome this problem, Dominique Siegenthaler, Benjamin Escribano and Vanessa Bräuler in the group of Professor Jan Pielage of TUK’s Division of Neurobiology and Zoology developed a genetic tag that responds to memory formation. The tag is called CRE-activity dependent memory engram label, or CAMEL, for short. It utilizes the DNA-binding site of the CRE binding protein (CREB2), which is essential for the formation of long-term memories. The tag lights up when neurons have an active CREB protein binding to DNA, highlighting only the neurons in the process of forming a memory.
“For the first time, we can specifically control the neurons forming long-term memories in Drosophila,” Pielage said.
As if seeing memory formation in action was not exciting enough, the tag does much more. The researchers can also use it to inhibit or activate the neurons’ activities.
“Now we have genetic access to get after the cellular and molecular changes occurring during long term memory formation,” Pielage said.
For example, the team used their tag to inhibit signaling from the long-term memory neurons in fruit flies. They trained the flies to associate an odor with an electric shock. The flies were still able to learn and recall memory in the first three hours after training, indicating that short-term memory formation was not affected by silencing these neurons. However, the flies were unable to recall the memory for the next four days, the time period when the CREB protein makes and maintains long-term memory. Interestingly, seven days after training, when CREB and thus the tag was no longer active, the flies again avoided the odor. This demonstrated that silencing CAMEL neurons specifically disrupted long-term memory recall but not production or storage of the memory.
To see if memories could be turned on artificially, the group tried a different experiment. They trained flies to associate an odor with a shock and then used their tag to express a channel that activates neurons when exposed to red light. When giving the flies the choice to move either in areas with or without red light, more flies chose to move to dark areas. This indicated that red light activated the neurons containing the “bad” memory about the odor and electric shock.
“The flies essentially avoided recalling this bad memory,” Pielage said.
Taken together, these experiments indicate that this small subset of tagged neurons is sufficient and required to make long-term memories, Pielage explained. The team next plans to create artificial memories in the flies, in order to systematically investigate the cellular changes that happen when creating and recalling memories.
“We still don’t know what happens in the neurons to store long-term memories or how we can recall memories,” Pielage said.
Original publication
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