The biological role of europium
Huub Op den Camp
In collaboration with microbiologists led by Huub Op den Camp at Radboud University in Nijmegen, Netherlands and Thomas Barends at the Max Planck Institute for Medical Research in Heidelberg, Lena Daumann (Professor for Bioinorganic Chemistry at the LMU Munich) and her group have now provided the first biochemical characterization of a europium-containing enzyme. This enzyme was purified from the bacterial strain M. fumariolicum SolV which was grown in the presence of europium as the sole REE in the medium. The isolated enzyme is a so called methanol dehydrogenase (MDH) which catalyzes the conversion of methanol into formaldehyde. Its active site consists of the cofactor pyrroloquinoline quinone (PQQ) and a central metal ion. In contrast to the long known calcium-dependent MDH, this SolV-derived enzyme holds a REE (in this study europium), in its active site.
The interdisciplinary research team not only solved the crystal structure of this novel europium-containing MDH but also determined kinetic parameters in order to define the effects of various REE ions on the enzyme's activity. The results revealed that the type of REE bound in the active site has a considerable impact on MDH activity and in turn also on the growth rate of SolV.
Original publication
Bérénice Jahn, Dr. Arjan Pol, Henning Lumpe, Dr. Thomas R. M. Barends, Andreas Dietl, Carmen Hogendoorn, Prof. Dr. Huub J. M. Op den Camp, Prof. Dr. Lena J. Daumann; "Similar but Not the Same: First Kinetic and Structural Analyses of a Methanol Dehydrogenase Containing a Europium Ion in the Active Site": ChemBioChem; 2018
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Original publication
Bérénice Jahn, Dr. Arjan Pol, Henning Lumpe, Dr. Thomas R. M. Barends, Andreas Dietl, Carmen Hogendoorn, Prof. Dr. Huub J. M. Op den Camp, Prof. Dr. Lena J. Daumann; "Similar but Not the Same: First Kinetic and Structural Analyses of a Methanol Dehydrogenase Containing a Europium Ion in the Active Site": ChemBioChem; 2018
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