Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

Chromatography products and catalogs

Chromatography products

Protein Purification Handbooks Protein Purification Handbooks

Protein purification made easy with Cytiva manuals

Step-by-step instructions for beginners and experts
The development of generic purification strategies has revolutionized protein purification by offering speed and simplicity at all scales
handbooks
ÄKTA pure micro - enabling small sample volumes ÄKTA pure micro - enabling small sample volumes

Maximum separation performance with minimum sample volume

ÄKTA pure micro: Efficient solutions for micropreparative columns
High performance for increased resolution with minimized internal volumes ✓ Allows for fraction collection in small droplets ✓ Equip your ÄKTA pure 25M for micropreparative runs using our Micro kit ✓
chromatography systems
ÄKTA Protein Purification Systems – Easy to Use Benchtop Systems to Accelerate Daily Routines ÄKTA Protein Purification Systems – Easy to Use Benchtop Systems to Accelerate Daily Routines

Efficient protein purification on a laboratory scale

Automated and flexible chromatography systems for your research
Designed for purification of biomolecules, providing speed, performance, and flexibility in research and process development
HPLC systems
Fast & Efficient Protein Purification with Cytiva’s Self-Cleaving Resin Fast & Efficient Protein Purification with Cytiva’s Self-Cleaving Resin

Fast & Efficient Protein Purification with Cytiva’s Self-Cleaving Resin

Boost your lab's efficiency by simplifying purification with a tag-free solution
Speeds up purification processes with a single-step workflow ✓ Delivers high-purity, tag-free proteins ✓ Ideal for a wide range of protein types, including those lacking affinity partners ✓
chromatography resins
Frontansicht LenS3 Frontansicht LenS3

Precise macromolecule analysis from 2-50 nm for UHPLC and SEC

Improved sensitivity and signal quality with green laser - now measure molecular weight and size directly
MALS detector ✓ Direct characterization of macromolecules down to 2 nm Rg ✓ Highly sensitive thanks to green laser ✓ Can be coupled with HPLC and UHPLC ✓
MALS detectors
Tritec-Chromatographic Refrigerators for ÄKta Systems (HPLC Systems) Tritec-Chromatographic Refrigerators for ÄKta Systems (HPLC Systems)

Energy-efficient chromatography refrigerators for precise temperature control

Safe storage of your HPLC systems at -5 °C to 20 °C with modern touchscreen display
With the microprocessor-controlled temperature controller, you have a 100% accurate overview and can adjust and check every setting on the comfortable
chromatography refrigerators
ÄKTA go – FPLC made compact ÄKTA go – FPLC made compact

Effective protein separation in a small space

Compact FPLC system for precise and fast purification results
The compact system, with a footprint of only 335 × 464 mm, lets you more efficiently use precious bench and cold cabinet space
FLPC systems
ÄKTA pure – Flexible and Intuitive Protein Purification System ÄKTA pure – Flexible and Intuitive Protein Purification System

ÄKTA pure – Flexible and Intuitive Protein Purification System

The Design Allows You to Customize and Easily Upgrade Your System to Meet Your Research Applications
ÄKTA pure chromatography system allows for easy customization thanks to the design of hardware and software. Choose from more than 20 hardware options
HPLC systems
Fully automated semiprep or/and prep system for laboratory-scale purification; also as GMP Fully automated semiprep or/and prep system for laboratory-scale purification; also as GMP

Fully automated semiprep or/and prep system for laboratory-scale purification; also as GMP

High-performance and compact HPLC system for semi-preparative and preparative applications
flow rates from up to 5,000 mL/min ✓ binary, ternary and quaternary high- and low-pressure gradients ✓ Flow control by pressure or volume ✓
HPLC systems
PerkinElmer GC 2400 Platform PerkinElmer GC 2400 Platform

PerkinElmer GC 2400 Platform

The Smart behind Separations
SMART: offers remote connectivity functionality with the detachable touchscreen ✓ SIMPLIFIED: Smart sampling capabilities, status notifications and easy-to-maintain hardware ✓ SUSTAINABLE: Smart gas management helps reduce gas consumption ✓
gas chromatographs
High capacity gas Autosampler High capacity gas Autosampler

Awesome - Gas Autosampler for up to 360 Samples

High sample throughput and contamination-free results for reliable measurements
High sample throughput - tray with up to 360 sample vials ✓ Contamination-free due to flushing and evacuation of the sample system ✓ Reliable - almost maintenance-free thanks to the robust design ✓
autosamplers
Holistic Multi-Column Chromatography Solution Holistic Multi-Column Chromatography Solution

Holistic Multi-Column Chromatography Solution

chromatography systems
YMC HT Glass Columns YMC HT Glass Columns

YMC HT Glass Columns

chromatography columns
New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins

Innovative SEC technology for the life sciences

chromatography columns
The NGC Package Deal: Your CHROMATOGRAPHY System The NGC Package Deal: Your CHROMATOGRAPHY System

Innovative chromatography for precise results

HPLC systems

Chromatography brochures

Chromatography expert knowledge

Chromatography white papers

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Chromatography explained

Affinity chromatography

Affinity chromatography Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody , enzyme and substrate , or receptor and ligand . Affinity chromatography combines the size ...

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Chiral column chromatography

Chiral column chromatography Chiral column chromatography is a variant of column chromatography , where the stationary phase is chiral instead of achiral. The enantiomer s of the same compound then differ in affinity to the stationary phase, thus they exit the column at different times. The chir ...

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Chromatographic Separation Methods in Simple Terms

Separating mixtures of substances to analyze them qualitatively and quantitatively – these are the chromatographic methods you should know about ...

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Chromatography

Chromatography Chromatography (from Greek χρώμα:chroma, colour and γραφειν:"grafein" to write) is the collective term for a family of laboratory techniques for the separation of mixtures . It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates ...

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Column chromatography

Column chromatography Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The classical preparative chromatography column is a glass tube ...

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Fast protein liquid chromatography

Fast protein liquid chromatography Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography . It is very commonly used in biochemistry and enzymology . Columns used w ...

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Gas-liquid chromatography

Gas-liquid chromatography Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen , and the stationary phase is a microscopic layer of li ...

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Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography (GPC) is a separation technique based on hydrodynamic volume (size in solution). Molecules are separated from one another based on differences in molecular size. This technique is often used for polymer molecular weight determination. GP ...

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High performance liquid chromatography

High performance liquid chromatography High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry . It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixt ...

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Hydrophilic interaction liquid chromatography

Hydrophilic interaction liquid chromatography HILIC (HydrophILic Interaction Chromatography or Hydrophilic Interaction LIquid Chromatography) is a version of normal phase liquid chromatography a name suggested by Dr. Andrew Alpert in his 1990 paper on the subject (J. Chromatogr. 499 (1990) 177) whe ...

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Mixed-mode chromatography

Mixed-mode chromatography Mixed-mode chromatography is a type of chromatography . In liquid chromatography , several modes of separation are commonly applied to resolve a mixture of different molecules. Ion-exchange chromatography , normal phase chromatography , reversed phase chromatography , size ...

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Size exclusion chromatography

Size exclusion chromatography Size exclusion chromatography (SEC) is a chromatographic method in which particles are separated based on their size, or in more technical terms, their hydrodynamic volume . It is usually applied to large molecule s or macromolecular complexes such as proteins and ind ...

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Supercritical fluid chromatography

Supercritical fluid chromatography Supercritical Fluid Chromatography (SFC) is a robust and easy-to-use form of normal phase chromatography ideally suited to the analysis and purification of low to moderate molecular weight, thermally labile molecules. It is especially suited to the separation of ...

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The Separation Principle Behind Chromatography in Simple Terms

An overview of the physical and chemical basics and more ...

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Thin layer chromatography

Thin layer chromatography Thin layer chromatography (TLC) is a chromatography technique used to separate chemical compounds . It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel , aluminium oxide , or cellulose immobilised onto a flat, inert carrie ...

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