Zebrafish

For the venomous Australian coral reef fish which is also known as zebrafish, see red lionfish.

The zebrafish or zebra danio, Danio rerio, is a tropical fish belonging to the minnow family (Cyprinidae).^[1] It is a popular aquarium fish, frequently sold under the trade name zebra danio, and is also an important model organism.

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Taxonomy

The zebrafish is a derived member of the genus Danio. It has a sister group relationship with Danio kyathit.^[2]

Distribution

The zebrafish is native to the streams of South-eastern Himalayan region.^[2] The zebrafish arose in the Ganges region in Eastern India and is also native to Pakistan, Bangladesh, Nepal and Myanmar.^{[citation needed]} It commonly inhabits streams, canals, ditches, ponds and slow-moving to stagnant water bodies, including rice fields.^{[citation needed]}

Zebrafish have been introduced in Japan and the United States as well as Australia, where they are kept in aquariums. ^[1]. They have also been sighted in Colombia, presumably having escaped from an aquarium.

Description

The fish is named for its five uniform, pigmented, horizontal blue stripes on the side of the body, all of which extend to the end of the caudal fin. Males are torpedo shaped and have gold stripes between the blue stripes; females have a larger, whitish belly and have silver stripes instead of gold. The zebrafish grows to 3.8 cm, lives for around 5 years, and produces 300-500 eggs per spawning.

Varieties

Recently, transgenic zebrafish have become commercially available that express green fluorescent protein, red fluorescent protein, and yellow fluorescent protein. These fish are tradenamed GloFish. Other varieties include golden, sandy, longfin and leopard.

The Leopard danio, previously known as Danio frankeri, is a spotted colour morph of the zebrafish Danio rerio caused by a pigment mutation.^[3] Xanthistic forms of both the zebra and leopard pattern, along with long-finned varieties have been obtained via selective breeding programs for the aquarium trade.^[4]

Common Strains

• AB
• TÜ
• IN, for India
• WIK

Aquarium Care

Zebrafish are hardy fish and considered good for beginner aquarists. Their ease of keeping and breeding, beauty, price and broad availability may all contribute to their popularity. They thrive best at temperatures above 22 degrees celsius and below 27 degrees celsius. They also thrive as shoals of 6 or more, although they do interact well with other fish types in the Aquarium. However, they are susceptible to Oodinium, or Velvet disease, Microsporidia (Pseudoloma neurophilia),and mycobacterium species.

Model organism for development and genetics

  D. rerio are a common and useful model organism for studies of vertebrate development and gene function.^[2] They may supplement or replace higher vertebrate models, such as rats and mice. Pioneering work of George Streisinger at the University of Oregon established the zebrafish as a model organism; its importance was consolidated by large scale forward genetic screens (commonly referred to as the Tübingen/Boston screens). The scholarly journal Development devoted an issue to research using the fish in celebration of this landmark. ^[2] An online database of zebrafish genetic, genomic, and developmental information (ZFIN) has been established. D. rerio is one of the few species of fish to have been flown into space (see Animals in space).   Zebrafish embryonic development provides advantages over other vertebrate model organisms. Although the overall generation time of zebrafish is comparable to that of mice, zebrafish embryos develop rapidly, progressing from eggs to larvae in under three days. The embryos are large, robust, and transparent and develop externally to the mother, characteristics which all facilitate experimental manipulation and observation. ^[5] Their nearly constant size during early development facilitates simple staining techniques, and drugs may be administered by adding directly to the tank. Unfertilised eggs can be made to divide, and the two-celled embryo fused into a single cell, creating a fully homozygous embryo.

A common reverse genetics technique is to reduce gene expression or modify splicing in zebrafish using Morpholino antisense technology. Morpholino oligonucleotides are stable, synthetic macromolecules that contain the same bases as DNA or RNA; by binding to complementary RNA sequences, they reduce the expression of specific genes. The journal Genesis devoted an issue to research using Morpholino oligos^[3], mostly in D. rerio. Morpholino oligonucleotides can be injected into one cell of a zebrafish embryo after the 32-cell stage, producing an organism in which gene expression is reduced in only the cells descended from the injected cell. However, cells in the early embryo (<32 cells) are interpermeable to large molecules^{[4], [5]}, allowing diffusion of Morpholinos between cells. A known problem with gene knockdowns in zebrafish is that, because the genome underwent a duplication after the divergence of ray-finned fishes and lobe-finned fishes, it is not always easy to silence the activity one of the two gene paralogs reliably due to complementation by the other paralog.

Despite the complications of the zebrafish genome a number of commercially available global platforms for analysis of both gene expression by microarrays and promoter regulation using ChIP-on-chip exist.

Zebrafish have the ability to regenerate fins, skin, the heart and the brain (in larval stages). Zebrafish have also been found to regenerate photoreceptors and retinal neurons following injury. The mechanisms of this regeneration are unknown, but are currently being studied. Researchers frequently cut the dorsal and ventral tail fins and analyze their regrowth to test for mutations. This research is leading the scientific community in the understanding of healing/repair mechanisms in vertebrates.

Recent Developments

In December 2005, a study of the golden strain identified the gene responsible for the unusual pigmentation of this strain as SLC24A5, a solute carrier that appears to be required for melanin production, and confirmed its function with a Morpholino knockdown. The orthologous gene was then characterized in humans and a one base pair difference was found to segregate strongly between fair skinned Europeans and dark skinned Africans. ^[6] This study featured on the cover of the academic journal Science and demonstrates the power of zebrafish as a model organism in the relatively new field of comparative genomics.

In January 2007, Chinese researchers at Fudan University raised genetically modified fish that can detect estrogen pollution in lakes and rivers, showing environmental officials what waterways need to be treated for the substance, which is linked to male infertility. Song Houyan and Zhong Tao, two professors at Fudan's molecular medicine lab, spent three years cloning estrogen-sensitive genes and injecting them into the fertile eggs of zebrafish. The modified fish turn green if they are placed into water that is polluted by estrogen^[7].

On August 1, 2007, British researchers said they had successfully grown in the laboratory a type of adult stem cell found in the eyes of both fish and mammals that develops into neurons in the retina.

In future, these cells could be injected into the eye as a treatment for diseases such as macular degeneration, glaucoma and diabetes-related blindness, according to Astrid Limb of University College London's (UCL) Institute of Ophthalmology.

Damage to the retina -- the part of the eye that sends messages to the brain -- is responsible for most cases of sight loss.

"Our findings have enormous potential," Limb said. "It could help in all diseases where the neurons are damaged, which is basically nearly every disease of the eye."

Limb and her colleagues studied so-called Mueller glial cells in the eyes of people aged from 18 months to 91 years and found they were able to develop them into all types of neurons found in the retina.

They were also able to grow them easily in the lab, they reported in the journal Stem Cells.

The cells have already been tested in rats with diseased retinas, where they successfully migrated into the retina and took on the characteristics of the surrounding neurons. Now the team is working on the same approach in humans.^[8].

See also

• The subfamily Danionin
• GloFish
• List of freshwater aquarium fish species

References

Further reading

• Danio rerio (TSN 163699). Integrated Taxonomic Information System. Accessed on 12 November 2004.
• "Danio rerio". FishBase. Ed. Ranier Froese and Daniel Pauly. October 2004 version. N.p.: FishBase, 2004.
• Lambert, Derek J (1997). Freshwater Aquarium Fish. Edison, New Jersey: Chartwell Books, Page 19. ISBN 0-7858-0867-1. 
• Sharpe, Shirlie. Zebra Danio. Your Guide to Freshwater Aquariums. Retrieved on December 15, 2004.
• Kocher, Thomas D et al.. Zebrafish, Vol. 2, #3, 2005 Roundtable Discussion- "Fish Models for Studying Adaptive Evolution and Speciation,".
• The Zebrafish Information Network (ZFIN)