White spot syndrome

White spot syndrome (WSS) is a viral infection of penaeid shrimp. The disease is highly lethal and contagious, killing shrimps quickly. Outbreaks of this disease have wiped out within a few days the entire populations of many shrimp farms throughout the world.

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The disease is caused by a family of related viruses subsumed as the White Spot Syndrome Baculovirus Complex (WSSV) ^[1] and the disease caused by them as white spot syndrome (WSS) ^[2].

The first reported epidemic due to this virus is from Taiwan in 1992 ^[3], however, reports of losses due to white spot disease came from China in 1993 ^[4], where it led to a virtual collapse of the shrimp farming industry. This was followed by outbreaks in Japan and Korea in the same year, Thailand, India and Malaysia in 1994 and by 1996 it had severely affected East Asia and South Asia. In late 1995, it was reported in the USA, 1998 in Central and South America, 1999 in Mexico and in 2000 in the Philippines. Currently, it is known to be present in all shrimp growing regions except Australia.

The virus has a wide host range and is highly virulent and leads to mortality rates of 100% within days in the case of cultured penaeid shrimps. Most of the cultured penaeid shrimps (Penaeus monodon, Marsupenaeus japonicus, Litopenaeus vannamei, Fenneropenaeus indicus, etc.) are natural hosts of the virus. Several non-penaeid shrimps were also found to be severely infected during experimental challenges. Many crustaceans like crabs (Scylla spp., Portunus spp.), spiny lobsters (Panulirus spp.), crayfish (Astacus spp., Cherax spp., etc.) and freshwater prawn (Macrobrachium spp.) are reported to get infected with variable severities depending on the life stage of the host and presence of external stressors (temperature, salinity, bacterial diseases, pollutants, etc.).

Clinical signs of WSSV include a sudden reduction in food consumption, lethargy, loose cuticle and often reddish discolouration, and the presence of white spots of 0.5 to 2.0 mm in diameter on the inside surface of the carapace, appendages and cuticle over the abdominal segments.

WSSV is a rod-shaped double-stranded DNA virus, and the size of the enveloped viral particles have been reported to be 240-380 nm long and 70-159 nm dia and nucleocapsid core is 120-205 nm long and 95-165 nm dia. The virus has an outer lipid bilayer membrane envelope, sometimes with a tail like appendage at one end of the virion. The nucleocapsid consists of 15 conspicuous vertical helices located along the long axis, each helix has two parallel striations, composed of 14 globular capsomers, each of which is 8 nm in diameter ^[5]. White spots on the shell of infected shrimp under scanning electron microscope appear as large dome shaped spots on the carapace measuring 0.3 to 3 mm in diameter. Smaller white spots of 0.02 to 0.1 mm appear as linked spheres on the cuticle surface. Chemical composition of the spots is similar to the carapace, calcium forming 80-90% of the total material and it is suggested to have derived from abnormalities of the cuticular epidermis ^[6]. The complete DNA sequence of WSSV genome has been assembled into a circular sequence of 292,967 bp ^[7].

Transmission of the virus is mainly through oral ingestion and water borne routes in farms (horizontal transmission) and vertical transmission (from infected mother prawns) in case of shrimp hatcheries. The virus is present in the wild stocks of shrimp, especially in the coastal waters adjacent to shrimp farming regions in Asian countries, but mass mortalities of wild shrimps are yet to be observed.

Rapid and specific diagnosis of the virus is carried out using two step-nested polymerase chain reaction (PCR). Histopathological changes in infected shrimps include prominent intranuclear eosinophilic to basophilic inclusions in the infected cells and cellular degeneration with hypertrophied nuclei and chromatin margination in the cuticular epidermis, gill epithelium, antennal gland, haematopoeitic tissue, nervous tissue and connective tissue and cellular necrosis and detachment of intestinal epithelial tissue ^[8].

There are no available treatments for WSS, although a large number of disinfectants are widely used in shrimp farms and hatcheries to prevent an outbreak. Stocking of uninfected shrimp seeds and rearing them away from environmental stressors with extreme care to prevent contamination are useful management measures.

White spot syndrome virus (WSSV) does not infect human beings and eating infected shrimp (in any form) does not pose any hazard to human health.

References

1. ^ Non-Native Species Summaries: White Spot Syndrome Baculovirus Complex (WSBV). Gulf States Marine Fisheries Commission (2003). Retrieved on 30 June, 2005.
2. ^ Lightner, D. V. (1996). A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp. World Aquaculture Society, Baton Rouge, Louisiana, USA. 
3. ^ Chen, S. N. (1995). "Current status of shrimp aquaculture in Taiwan.", in C. L. Browdy & J. S. Hopkins (Eds.): Swimming through troubled water. Proceedings of the special session on shrimp farming. Aquaculture ’95. World Aquaculture Society, Baton Rouge, Louisiana, USA, pp 29-34. 
4. ^ Huang, J., X. L. Song, J. Yu & C. H. Yang (1995). Baculoviral hypodermal and hematopoietic necrosis: study on the pathogen and pathology of the explosive epidemic disease of shrimp. Marine Fisheries Research 16: 1-10.
5. ^ Nadala, E. C. B., Jr., L. M. Tapay & P. C. Loh (1998). Characterization of a non-occluded baculovirus-like agent pathogenic to penaeid shrimp. Diseases of Aquatic Organisms 33: 221- 229.
6. ^ Wang, C. S., K. F. Tang, G. H. Kou & S. N. Chen (1997). Light and electron microscopic evidence of white spot disease in the giant tiger shrimp, Penaeus monodon (Fabricius), and the kuruma shrimp, Penaeus japonicus (Bate), cultured in Taiwan. Journal of fish Diseases 20: 323-331.
7. ^ Van Hulten, M. C. W., J. Witteveldt, S. Peters, N. Kloosterboer, R. Tarchini, F. Fiers, H. Sandbrink, R. K. Lankhorst & J. M. Vlak (2001). The white spot syndrome virus DNA genome sequence. Virology 286: 7-22.
8. ^ Wongteerasupaya, C., J. E. Vickers, S. Sriurairatana, G. L. Nash, A. Akarajamorn, V. Boonseang, S. Panyin, A. Tassanakajon, B. Withyachumnarnkul & T. W. Flegel (1995). A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Diseases of Aquatic Organisms 21: 69-77.