To use all functions of this page, please activate cookies in your browser.
my.bionity.com
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
UltramicrotomyUltramicrotomy is a method for preparing a sample, often tissue embedded in resin, for examination in a transmission electron microscope (TEM). The sample block is first trimmed to create a block face 1 mm by 1 mm in size. "Thick" sections (1 μm) are taken to be looked at on an optical microscope. An area is chosen to be sectioned for the TEM and the block face is re-trimmed to a size no larger than 0.7 mm on a side. Block faces usually have a square, trapezoid, rectangle or triangle shape. Finally, thin sections are cut with a diamond or glass knife and the sections are left floating on water that is held in a boat or trough. The sections are then retrieved from the water surface and mounted on a copper, nickel or other metal grid. Ideal section thickness for transmission electron microscopy with accelerating voltages between 50 kV and 120 kV is about 50–100 nm. |
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Ultramicrotomy". A list of authors is available in Wikipedia. |