To use all functions of this page, please activate cookies in your browser.
my.bionity.com
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
Type I topoisomeraseType I topoisomerases function by nicking one of the strands of the DNA double helix, twisting it around the other strand, and re-ligating the nicked strand. This is not an active process in the sense that energy in the form of ATP is not spent during the nicking or ligation steps as the reaction between the tyrosine residue at the active site of the enzyme with the phosphodiester DNA backbone simply replaces one phosphomonoester bond with another. The topoisomerase also does not use ATP during uncoiling of the DNA; rather, the torque present in the DNA drives the uncoiling. Additional recommended knowledge
ClassificationType I enzymes can be further subdivided into type IA and type IB, based on their chemistry of action.
Prokaryotic topoisomerase I can only relax negative supercoiling, whereas eukaryotic topoisomerase I can affect both negative and positive supercoiling. These variants of topoI are not evolutionarily related. IntermediatesAll topoisomerases form a phosphotyrosine intermediate between the catalytic tyrosine of the enzyme and the scissile phosphoryl of the DNA backbone.
DiversityApart from these similarities, they have very different mechanisms of action, have different crystal structures and appear not to have similar evolutionary ancestors. InhibitionType 1 topoisomerase is inhibited by irinotecan, topotecan and camptothecin.
|
|||
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Type_I_topoisomerase". A list of authors is available in Wikipedia. |