TMB Liquid substrate for ELISA

Enzyme Linked Immuno Sorbent Assay (ELISA) is a widely used method for both research and diagnostic purposes. It is an assay where the reaction between the antigen and its antibody takes place linked to a solid phase. For visualization of the antigen-antibody reaction an enzyme and the related substrate offer a coloured reaction which can be measured spectrophotometrically. Different substrates are available to the enzyme conjugates used in ELISA. TMB (3, 3’, 5, 5’-tetramethylbenzidine) is the most commonly used substrate for the enzyme horseradish peroxidase (HRP) and the combination is one of the most acknowledged ELISA detection systems. ’’Mono-component TMB systems combine TMB, substrate buffer and hydrogen peroxide for safe, ready-to-use, one step products for direct use in your preferred ELISA format.

Additional recommended knowledge

Daily Sensitivity Test

Weighing the right way

How to ensure accurate weighing results every day?

TMB yields a soluble blue end product read at 655 nm. The reaction may be stopped by 0.2 M sulphuric acid which offers a yellow end product read at 450 nm. Ready-to-use TMB substrates are available in different activity levels to match individual requirements. All types of ELISA TMB products are available also as prestained, colour coded products for maximum convenience.

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