SYBR Green

Additional recommended knowledge

Safe Weighing Range Ensures Accurate Results

Weighing the right way

Essential Laboratory Skills Guide

SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. SYBR Green I binds to double-stranded DNA. The resulting DNA-dye-complex absorbs blue light (λ_max = 488 nm) and emits green light (λ_max = 522 nm). SYBR Green I could replace the very strong mutagen ethidium bromide.

Similar cyanine dyes:

• SYBR Green II
• SYBR Gold
• YO (Oxazole Yellow)
• TO (Thiazole Orange)
• PG (PicoGreen)

Literature

Zipper, H. et al. (2004). Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res. 32, e103. PMID 15249599 PDF