R2a agar

R2A agar is a culture medium developed to study bacteria which normally inhabit potable water. These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium. Since its development in 1979, it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media.

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Typical Composition (g/l)

Proteose peptone, 0.5
Casamino acids, 0.5
Yeast extract, 0.5
Glucose, 0.5
Soluble starch, 0.5
Dipotassium phosphate, 0.3
Magnesium sulfate 7H₂O, 0.05
Sodium pyruvate, 0.3
Agar, 15
Final pH 7.2 ± 0.2 @ 25 °C