Purkinje cell
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Drawing of pigeon Purkinje cells (A) by Santiago Ramon y Cajal
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Location
| Cerebellum
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Function
| inhibitory projection neuron
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Morphology
| flat dendritc arbor
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Presynaptic connections
| Parallel fibers and Climbing fibers
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Postsynaptic connections
| Cerebellar deep nuclei
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For the cells of the electrical conduction system of the heart, see Purkinje fibers
Purkinje cells (or Purkinje neurons) are a class of GABAergic neuron located in the cerebellar cortex. They are named after their discoverer, Czech anatomist Jan Evangelista Purkyně.
Additional recommended knowledge
Anatomy
These cells are some of the largest neurons in the human brain, with an intricately elaborate dendritic arbor, characterized by a large number of dendritic spines. Purkinje cells are found within the Purkinje layer in the cerebellum. Purkinje cells are aligned like dominos stacked one in front of the other. Their large dendritic arbors form nearly two dimensional layers through which parallel fibers from the deeper-layers pass. These parallel fibers make relatively weaker excitatory (glutamatergic) synapses to spines in the Purkinje cell dendrite, whereas climbing fibers originating from the inferior olivary nucleus in the medulla provide very powerful excitatory input to the proximal dendrites and cell soma. Parallel fibers pass orthogonally through the Purkinje neuron's dendritic arbor, with up to 200,000 parallel fibers forming a synapse with a single Purkinje cell. Alternatively, each Purkinje cell only receives a synapse from a single climbing fiber. Both basket and stellate cells (found in the cerebellar molecular layer) provide inhibitory (GABAergic) input to the Purkinje cell, with basket cells synapsing on the Purkinje cell axon initial segment and stellate cells onto the dendrites.
Purkinje cells send inhibitory projections to the deep cerebellar nuclei, and constitute the sole output of all motor coordination in the cerebellar cortex.
Electrophysiological activity
Purkinje cells show two distinct forms of electrophysiological activity:
- Simple spikes occur at rates of 50 - 150 Hz either spontaneously or and when Purkinje cells are activated synaptically by the parallel fibers, the axons of the granule cells.
- Complex spikes are rapid (>300 Hz) bursts of spikes caused by climbing fiber activation, and can involve the generation of calcium-mediated action potentials in the dendrites. Following complex spike activity simple spikes can be suppressed by the powerful complex spike input.
Purkinje cells show spontaneous electrophysiological activity in the form of trains of spikes both sodium as well as calcium dependent was initially shown by Rodolfo Llinas (Llinas and Hess (1977) and Llinas and Sugimori (1980. P type calcium channels were named after Purkinje cells where they were initially encountered (Llinas et al 1989), which are crucial in cerebellar function. It has recently been shown that climbing fiber activation of the Purkinje cell can shift its activity from a quiet state to a spontaneously active state, and vice-versa, serving as a type of toggle switch (Lowenstein et al., 2005, Nature Neuroscience). However, these findings have recently been challenged by a study suggesting that such toggling by climbing fiber inputs occurs predominantly in anaesthetized animals, and that Purkinje cells in awake behaving animals in general operate almost continuously in the upstate (Schonewille et al., 2006, Nature Neuroscience).
Findings have suggested that Purkinje cell dendrites release endocannabinoids that can transiently downregulate both excitatory and inhibitory synapses[1]
Medical conditions related to Purkinje cells
In humans, Purkinje cells are affected in a variety of diseases ranging from toxic exposure (alcohol, lithium), to autoimmune diseases and to genetic mutations (spinocerebellar ataxias, autism) and neurodegenerative diseases that are not thought to have a known genetic basis (cerebellar type of multiple system atrophy, sporadic ataxias).
In some domestic animals, a condition where the Purkinje cells begin to atrophy shortly after birth, called Cerebellar abiotrophy, can lead to symptoms including ataxia, intention tremors, hyperreactivity, lack of menace reflex, stiff or high-stepping gait, apparent lack of awareness of where the feet are (sometimes standing or walking with a foot knuckled over), and a general inability to determine space and distance.[citation needed] A similar condition known as cerebellar hypoplasia occurs when Purkinje cells either fail to develop in utero or die off in utero prior to birth. Ataxia-Telangiectasia is a genetic condition, in which Purkinje cells are progressively lost.
Links
- Cerebellar disoders
- Disorders of cerebellum
References
- Llinas R. and Hess, R. (1976) Proc. Natl. Acad. Science US 73: 2520-2523.
- Llinas R. and Sugimori M. (1980) J. Physiol. 305: 171-195
- Llinas R. Sugimori M. and Cherksey B. (1989) Annals of the New York Acad. Science 560:103-111
- ^ Kreitzer A & Regehr W, 2001. Retrograde Inhibition of Presynaptic Calcium Influx by Endogenous Cannabinoids at Excitatory Synapses onto Purkinje Cells. Neuron Mar;29(3):717-27.
Histology: nervous tissue |
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Neurons (gray matter) | soma, axon (axon hillock, axoplasm, axolemma, neurofibril/neurofilament), dendrite (Nissl body, dendritic spine, apical dendrite, basal dendrite) types (bipolar, pseudounipolar, multipolar, pyramidal, Purkinje, granule) |
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Afferent nerve/Sensory nerve/Sensory neuron | GSA, GVA, SSA, SVA, fibers (Ia, Ib or Golgi, II or Aβ, III or Aδ or fast pain, IV or C or slow pain) |
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Efferent nerve/Motor nerve/Motor neuron | GSE, GVE, SVE, Upper motor neuron, Lower motor neuron (α motorneuron, γ motorneuron) |
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Synapses | neuropil, synaptic vesicle, neuromuscular junction, electrical synapse - Interneuron (Renshaw) |
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Sensory receptors | Free nerve ending, Meissner's corpuscle, Merkel nerve ending, Muscle spindle, Pacinian corpuscle, Ruffini ending, Olfactory receptor neuron, Photoreceptor cell, Hair cell, Taste bud |
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Glial cells | astrocyte, oligodendrocyte, ependymal cells, microglia, radial glia |
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Myelination (white matter) | Schwann cell, oligodendrocyte, nodes of Ranvier, internode, Schmidt-Lanterman incisures, neurolemma |
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Related connective tissues | epineurium, perineurium, endoneurium, nerve fascicle, meninges |
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Brain: rhombencephalon (hindbrain) |
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Myelencephalon/medulla |
anterior/ventral: Arcuate nucleus of medulla • Pyramid (Decussation) • Olivary body • Inferior olivary nucleus • Anterior median fissure • Ventral respiratory group
posterior/dorsal: VII,IX,X: Solitary/tract • XII, X: Dorsal • IX,X,XI: Ambiguus • IX: Inferior salivatory nucleus • Gracile nucleus/Cuneate nucleus/Accessory cuneate nucleus • Area postrema • Posterior median sulcus • Dorsal respiratory group
raphe/reticular: Sensory decussation • Reticular formation (Gigantocellular nucleus, Parvocellular reticular nucleus, Ventral reticular nucleus, Lateral reticular nucleus, Paramedian reticular nucleus) • Raphe nuclei (Obscurus, Magnus, Pallidus)
tracts: Corticospinal tract (Lateral, Anterior) • Inferior cerebellar peduncle • Olivocerebellar tract • Spinocerebellar (Dorsal, Ventral) • Spinothalamic tract • PCML (Posterior external arcuate fibers, Internal arcuate fibers, Medial lemniscus) • Extrapyramidal (Rubrospinal tract, Vestibulospinal tract, Tectospinal tract)
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Metencephalon/pons |
anterior/ventral: Superior olivary nucleus • Basis pontis (Pontine nuclei, Middle cerebellar peduncles)
posterior/dorsal: Pontine tegmentum (Trapezoid body, Superior medullary velum, Locus ceruleus, MLF, Vestibulocerebellar tract, V Principal Spinal & Motor, VI, VII, VII: Superior salivary nucleus) • VIII-c (Dorsal, Anterior)/VIII-v (Lateral, Superior, Medial, Inferior)
raphe/reticular: Reticular formation (Caudal pontine reticular nucleus, Oral pontine reticular nucleus, Tegmental pontine reticular nucleus, Paramedian pontine reticular formation) • Median raphe nucleus
Apneustic center • Pneumotaxic center
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Metencephalon/cerebellum | Vermis • Flocculus • Arbor vitae • Cerebellar tonsil • Inferior medullary velum
Molecular layer (Stellate cell, Basket cell, Parallel fiber) • Purkinje cell layer (Purkinje cell) • Granule cell layer (Golgi cell) • Mossy fibers • Climbing fiber
Deep cerebellar nuclei (Dentate, Emboliform, Globose, Fastigial) |
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Fourth ventricle | apertures (Median, Lateral) • Rhomboid fossa (Vagal trigone, Hypoglossal trigone, Obex, Sulcus limitans, Facial colliculus, Medial eminence) • Lateral recess |
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