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Protein methods

Protein methods are the techniques used to study proteins.

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1 List of Protein Methods
2 Other methods
3 References

List of Protein Methods

There are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying proteins and other methods for characterizing the structure and function of proteins, often requiring that the protein first be purified.

Genetic methods

conceptual translation- many proteins are never directly sequenced, but their sequence of amino acids is known by "conceptual translation" of a known mRNA sequence. See Genetic code.
site-directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function.
- insertion of protein tags such as the His-tag. See also: Green fluorescent protein.
evolutionary; analysis of sequence changes in different species using software such as BLAST.
Proteins that are involved in human diseases can be identified by matching alleles to disease and other phenotypes using methods such as calculation of LOD scores.

Detecting proteins

microscopy, Protein immunostaining
immunoprecipitation, Protein immunoprecipitation
Western blot
Spectrophotometry
Enzyme assay

Protein purification

Protein Isolation
- chromatography methods
Protein Extraction and Solubilization
Protein Concentration Determination Methods, Bradford protein assay
Concentrating Protein Solutions
Gel electrophoresis
- Gel Electrophoresis Under denaturing conditions
- Gel Electrophoresis Under non-denaturing conditions
- 2D Gel Electrophoresis
Electrofocusing

Protein structures

Other methods

Hydrogen-deuterium exchange
Mass spectrometry
Molecular dynamics
Protein structure prediction
Protein sequencing
Protein structural alignment
Protein ontology
Peptide mass fingerprinting
Ligand binding assay
metabolic labeling
- heavy isotope labeling
- radioactive isotope labeling

References

"Protein Methods", 2nd Edition by Daniel M. Bollag, Michael D. Rozycki and Stuart J. Edelstein (1996) Published by Wiley Pblishers ISBN 0-471-11837-0.

v • d • e Proteins
Protein biosynthesis - Posttranslational modification - Protein folding - Protein structure - Protein structural domains - Protein targeting - Proteome - Protein methods - Proteasome List of types of proteins - List of proteins - Membrane protein - Globular protein - Fibrous protein

v • d • e Proteins: key methods of study
Experimental	Protein purification - Green fluorescent protein - Western blot - Protein immunostaining - Protein sequencing - Gel electrophoresis/Protein electrophoresis - Protein immunoprecipitation - Peptide mass fingerprinting
Bioinformatics	Protein structure prediction - Protein-protein docking - Protein structural alignment - Protein ontology - Protein-protein interaction prediction
Assay	Enzyme assay - Protein assay - Secretion assay

Category: Protein methods

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Protein_methods". A list of authors is available in Wikipedia.