Phytochrome

Phytochrome is a photoreceptor, a pigment that plants use to detect light. It is sensitive to light in the red and far-red region of the visible spectrum. Many flowering plants use it to regulate the time of flowering based on the length of day and night (photoperiodism) and to set circadian rhythms. It also regulates other responses including the germination of seeds, elongation of seedlings, the size, shape and number of leaves, the synthesis of chlorophyll, and the straightening of the epicotyl or hypocotyl hook of dicot seedlings.

Biochemically, phytochrome is a protein with a bilin chromophore.

Phytochrome has been found in most plants including all higher plants; very similar molecules have been found in several bacteria. A fragment of a bacterial phytochrome now has a solved three-dimensional protein structure.

Other plant photoreceptors include cryptochromes and phototropins, which are sensitive to light in the blue and ultra-violet regions of the spectrum.

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Isoforms or states

Phytochromes are characterised by a red/far-red photochromicity. Photochromic pigments change their "colour" (spectral absorbance properties) upon light absorption. In the case of phytochrome the ground state is P_r, the _r indicating that it absorbs red light particularly strongly. The absorbance maximum is a sharp peak 650–670 nm, so concentrated phytochrome solutions look turquoise-blue to the human eye. But once a red photon has been absorbed, the pigment undergoes a rapid conformational change to form the P_fr state. Here _fr indicates that now not red but far-red (also called near infra-red; 705–740 nm) is preferentially absorbed. This shift in absorbance is apparent to the human eye as a slightly more greenish colour. When P_fr absorbs far-red light it is converted back to P_r. Hence, red light makes P_fr, far-red light makes P_r. In plants at least P_fr is the physiologically active or "signalling" state. Summary of the characteristics of plant phytochromes.

Purified Cph1 phytochrome in the Pr state (left) and the Pr/Pfr mixture (right) that is formed by irradiation with red light. Since daylight contains a lot of red light, during the day phytochrome is mostly converted to P_fr. At night, phytochrome will slowly convert back to the P_r form. Treatment with far-red light will also convert P_fr back to P_r. Since plants use red light for photosynthesis, and reflect and transmit far-red light, the shade of other plants also can make P_fr into P_r, triggering a response called shade avoidance. In most plants, a suitable concentration of P_fr stimulates or inhibits physiological processes, such as those mentioned in these examples.

Since both the ground state P_r and excited state P_fr are unusually stable (P_fr has a half-life of hours or days) the quantum nature of this transition was not immediately recognized. These two forms are therefore commonly (though technically incorrectly) referred to as isoforms.

Biochemistry

Chemically, phytochrome consists of a chromophore, a single bilin molecule consisting of an open chain of four pyrrole rings, bonded to the protein moiety. It is the chromophore that absorbs light, and as a result changes the conformation of bilin and subsequently that of the attached protein, changing it from one state or isoform to the other.

The phytochrome chromophore is usually phytochromobilin, and is closely related to phycocyanobilin (the chromophore of the phycobiliproteins used by cyanobacteria and red algae to capture light for photosynthesis) and to the bile pigment bilirubin (whose structure is also affected by light exposure, a fact exploited in the phototherapy of jaundiced newborns). The term "bili" in all these names refers to bile. Bilins are derived from the closed tetrapyrrole ring of haem by an oxidative reaction catalysed by haem oxygenase to yield their characteristic open chain. Chlorophyll too is derived from haem. In contrast to bilins, haem and chlorophyll carry a metal atom in the center of the ring, iron or magnesium, respectively.

The P_fr state passes on a signal to other biological systems in the cell, such as the mechanisms responsible for gene expression. Although this mechanism is almost certainly a biochemical process, it is still the subject of much debate. It is known that although phytochromes are synthesized in the cytosol and the P_r form is localized there, the P_fr form, when generated by light illumination, is translocated to the cell nucleus. This implies a role of phytochrome in controlling gene expression, and many genes are known to be regulated by phytochrome, but the exact mechanism has still to be fully discovered. It has been proposed that phytochrome, in the P_fr form, may act as a kinase, and it has been demonstrated that phytochrome in the P_fr form can interact directly with transcription factors.

Discovery

The phytochrome pigment was discovered by Sterling Hendricks and Harry Borthwick at the USDA-ARS Beltsville Agricultural Research Center in Maryland during a period from the late 1940s to the early 1960s. Using a spectrograph built from borrowed and war-surplus parts, they discovered that red light was very effective for promoting germination or triggering flowering responses. The red light responses were reversible by far-red light, indicating the presence of a photoreversible pigment.

The phytochrome pigment was identified using a spectrophotometer in 1959 by biophysicist Warren Butler and biochemist Harold Siegelman. Butler was also responsible for the name, phytochrome.

In 1983 the laboratories of Peter Quail and Clark Lagarias reported the chemical purification of the intact phytochrome molecule, and in 1985 the first phytochrome gene sequence was published by Howard Hershey and Peter Quail. By 1989, molecular genetics and work with monoclonal antibodies that more than one type of phytochrome existed; for example, the pea plant was shown to have at least two phytochrome types (then called type I (found predominantly in dark-grown seedlings) and type II (predominant in green plants)). It is now known by genome sequencing that Arabidopsis has five phytochrome genes (PHYA - E) but that rice has only three (PHYA - C). While this probably represents the condition in several di- and monocotyledonous plants, many plants are polyploid. Hence maize, for example, has six phytochromes - phyA1, phyA2, phyB1, phyB2, phyC1 and phyC2. While all these phytochromes have significantly different protein components, they all use phytochromobilin as their light-absorbing chromophore. In the late 1980s, the Vierstra lab showed that phyA is degraded by the ubiquitin system, the first identified natural target of the system to be identified in eukaryotes.

In 1996 a gene in the newly sequenced genome of the cyanobacterium Synechocystis was noticed to have a weak similarity to those of plant phytochromes. Jon Hughes in Berlin and Clark Lagarias at UC Davis subsequently showed that this gene indeed encoded a bona fide phytochrome (named Cph1) in the sense that it is a red/far-red reversible chromoprotein. Presumably plant phytochromes are derived from an ancestral cyanobacterial phytochrome, perhaps by gene migration from the chloroplast to the nucleus. Subsequently phytochromes have been found in other prokaryotes including Deinococcus radiodurans and Agrobacterium tumefaciens. In Deinococcus phytochrome regulates the production of light-protective pigments, however in Synechocystis and Agrobacterium the biological function of these pigments is still unknown.

In 2005, the Vierstra and Forest labs at the University of Wisconsin published a three-dimensional structure of the photosensory domain of Deinococcus phytochrome. This breakthrough paper revealed that the protein chain forms a knot - a highly unusual structure for a protein.

Genetic engineering

Around 1989 several laboratories were successful in producing (transgenic plants) which produced elevated amounts of different phytochromes (overexpression). In all cases the resulting plants had conspicuously short stems and dark green leaves. Harry Smith and coworkers at Leicester University in England showed that by increasing the expression level of phytochrome A (which responds to far-red light) shade avoidance responses can be altered. As a result, plants can expend less energy on growing as tall as possible and have more resources for growing seeds and expanding their root systems. This could have many practical benefits: for example, grass blades that would grow more slowly than regular grass would not require mowing as frequently, or crop plants might transfer more energy to the grain instead of growing taller.

References

• http://www.ars.usda.gov/is/timeline/light.htm
• http://www.mobot.org/jwcross/duckweed/phytochrome.htm#tetrapyrrole
• http://ucce.ucdavis.edu/files/filelibrary/616/17562.htm
• Terry and Gerry Audesirk. Biology: Life on Earth.
• Linda C Sage. A pigment of the imagination: a history of phytochrome research. Academic Press 1992. ISBN 0-12-614445-1