Physarum polycephalum

Physarum polycephalum belongs to phylum Amoebozoa, infraphylum Mycetozoa, and class Myxogastrea. P. polycephalum, often referred to as the “many-headed slime,” is a slime mold that inhabits shady, cool, moist areas, such as decaying leaves and logs.

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Characteristics

This protist may be seen without a microscope; P. polycephalum is typically yellow in color, and eats fungal spores, bacteria, and other microbes. P. polycephalum is one of the easiest eukaryotic microbes to grow in culture, and has been used as a model organism for many studies involving amoeboid movement and cell motility.

Life cycle

The main vegetative phase of P. polycephalum is the plasmodium (the active, streaming form of slime molds). The plasmodium is comprised of networks of protoplasmic veins, and many nuclei. It is during this stage that the organism searches for food. The plasmodium surrounds its food and secretes enzymes to digest it.

If environmental conditions cause the plasmodium to desiccate during feeding or migration, Physarum will form a sclerotium. The sclerotium is basically hardened multinucleated tissue that serves as a dormant stage, protecting Physarum for long periods of time. Once favorable conditions resume, the plasmodium reappears to continue its quest for food.

As the food supply runs out, the plasmodium stops feeding and begins its reproductive phase. Stalks of sporangia form from the plasmodium; it is within these structures that meiosis occurs and spores are formed. Sporangia are usually formed in the open so that the spores they release will be spread by wind currents.

Spores can remain dormant for years if need be. However, when environmental conditions are favorable for growth, the spores germinate and release either flagellated or amoeboid swarm cells (motile stage); the swarm cells then fuse together to form a new plasmodium.

Streaming behavior

The movement of P. polycephalum is termed shuttle streaming. Shuttle streaming is characterized by the rhythmic back-and-forth flow of the protoplasm; the time interval is approximately two minutes. The forces of the streaming vary for each type of microplasmodium.

The force in amoeboid microplasmodia is generated by contraction and relaxation of a membranous layer probably consisting of actin (type of filament associated with contraction). The filament layer creates a pressure gradient, over which the protoplasm flows within limits of the cell periphery.

The force behind streaming in the dumbbell-shaped microplasmodia is generated by volume changes in both the periphery of the cell and in the invagination system of the cell membrane.

Sources

• Gawlitta,W, KV Wolf, HU Hoffmann, and W. Stockem. 1980. Studies on microplasmodia of Physarum polycephalum. I. Classification and locomotive behavior. Cell Tissue Res; 209(1): 71-86.
• http://www.carolina.com/tips/01pdfs/August_2001_Tips.pdf
• http://www.educationalassistance.org/Physarum/PhysarumPlus.html