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Phenol extraction



Phenol extraction is a method used to purify a DNA sample.

In this method, a mixture of TE (or Tris-Ethylenediaminetetraacetic acid) and phenol is combined with an equal volume of an aqueous DNA sample. After agitation and centrifugal separation, the aqueous layer is extracted, and further processed with ether. Then the DNA is concentrated by ethanol precipitation.

See also

References

  • http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/phenol.html


 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Phenol_extraction". A list of authors is available in Wikipedia.
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