Peristimulus time histogram

In neurophysiology, peristimulus time histogram and poststimulus time histogram, both abbreviated PSTH or PST histogram, are histograms of the times at which neurons fire. These histograms are used to visualize the rate and timing of neuronal spike discharges in relation to an external stimulus or event. The peristimulus time histogram is sometimes called perievent time histogram, and post-stimulus and peri-stimulus are often hyphenated.

Additional recommended knowledge

Correct Test Weight Handling Guide: 12 Practical Tips

Daily Sensitivity Test

Weighing the right way

The prefix peri, for through, is typically used in the case of periodic stimuli, in which case the PSTH show neuron firing times wrapped to one cycle of the stimulus. The prefix post is used when the PSTH shows the timing of neuron firings in response to a stimulus event or onset.^[1]

To make a PSTH, a spike train recorded from a single neuron is aligned with the onset, or a fixed phase point, of an identical stimulus repeatedly presented to an animal. The aligned sequences are superimposed in time, and then used to construct a histogram.^[2]

Construction procedure

Align spike sequences with the onset of stimuli that repeated n times. For periodic stimuli, wrap the response sequence back to time zero after each time period T, and count n as the total number of periods of data.
Divide the stimulus period or observation period T into N bins of size $Δ$ .
Count the number of spikes k_i from all n sequences that fall in the bin i.
Draw a bar-graph histogram with the bar-height of bin i given by $\frac{k_i}{n \Delta}$ in units of estimated spikes per second at time $i\ \Delta$ .

References

^ Franklin Bretschneider and Jan R. De Weille (2006). Introduction to Electrophysiological Methods and Instrumentation. Academic Press. ISBN 0123705886.
^ Palm, G.; Aertsen, A. M.; Gerstein, G. L. (1988). "On the significance of correlations among neuronal spike trains". Biological Cybernetics 59 (1): 1-11.