NtrC

NtrC is the name of the protein necessary for the procaryotic regulon transcription factor sigma N (sigma 54) to form an open complex with RNA polymerase in order to activate glnA transcription. The open --> closed conformational change of the sigmaN-RNA polymerase complex around the glutamine synthetase gene promoter requires ATP and involves the 180 degree turn of the DNA so that the NtrC protein (bound to two -140 and -108 upstream enhancer regions) may interact directly with the promoter-bound sigma N-RNA polymerase complex.

Additional recommended knowledge

Essential Laboratory Skills Guide

Don't let static charges disrupt your weighing accuracy

How to ensure accurate weighing results every day?

References

Lecture notes, Molecular Genetics 3 at Edinburgh University autumn 2006