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Nile red



Nile red
IUPAC name Nile red
Other names Nile blue oxazone
Identifiers
CAS number 7385-67-3
PubChem 65182
SMILES CCN(CC)C1=CC2=C(C=C1)N= C3C4=CC=CC=C4C(=O)C=C3O2
Properties
Molecular formula C20H18N2O2
Molar mass 318.369 g/mol
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references

Nile red (also known as Nile blue oxazone) is a lipophilic stain. It is produced by boiling a solution of Nile blue with sulfuric acid. As can be seen from the structural formulae, this process replaces an amino group with a carbonyl group. Nile red stains intracellular lipid droplets red. Nile red is also intensely fluorescent, with a strong yellow-gold emission when in a lipid-rich environment.

Since the reaction to generate Nile red does not usually completely exhaust the supply of Nile blue, additional separation steps are required if pure Nile red is needed.

External links
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Nile_red". A list of authors is available in Wikipedia.
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