Multiplicity of infection

The multiplicity of infection or MOI is the ratio of infectious agents (e.g. phage or virus) to infection targets (e.g. cell). For example, when referring to a group of cells inoculated with infectious virus particles, the multiplicity of infection or MOI is the ratio defined by the number of infectious virus particles deposited in a well divided by the number of target cells present in that well.

Additional recommended knowledge

Essential Laboratory Skills Guide

Safe Weighing Range Ensures Accurate Results

Daily Sensitivity Test

Interpretation

The actual number of phages or viruses that will enter any given cell is a statistical process: some cells may absorb more than one virus particle while others may not absorb any. The probability that a cell will absorb n virus particles when inoculated with an MOI of m can be calculated for a given population using a Poisson distribution.

where m is the multiplicity of infection or MOI, n is the number of infectious agents that enter the infection target, and P(n) is the probability that an infection target (a cell) will get infected by n infectious agents.

For example, when an MOI of 1 (1 viral particle per cell) is used to infect a population of cells, the probability that a cell will not get infected is P(0) = 36.79%, and the probability that it be infected by a single particle is P(1) = 36.79%, by two particles is P(2) = 18.39%, by three particles is P(3) = 6.13%, and so on.

The average percentage of cells that will become infected as a result of inoculation with a given MOI can be obtained by realizing that it is simply P(n > 0) = 1 − P(0). Hence, the average fraction of cells that will become infected following an inoculation with an MOI of m is given by:

which is approximately equal to m for small values of .

See also

• LD50
• Infectious disease