To use all functions of this page, please activate cookies in your browser.
my.bionity.com
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
Minicircle
Additional recommended knowledgeMinicircles are small (~4kb) circular plasmid derivatives that have been freed from all prokaryotic vector parts. For uses as transgene carriers for the genetic modification of mammalia cells, their preparation usually follows a two-step procedure:
The purified minicircle can be transferred into the recipient cell by transfection or lipofection and into a differentiated tissue, for instance, by jet injection. A novel addition to the field are self-replicating minicircles, which owe this property to the presence of a S/MAR-Element. Self-replicating minicircles hold great promise for the systematic modification of stem cells the more as the feasibility of such an approach has recently been demonstrated with their precursor form (Ref.) See also
References
Categories: Genetics | Molecular genetics |
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Minicircle". A list of authors is available in Wikipedia. |