Jurkat cells

Jurkat cells are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia and T cell signaling. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.

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The Jurkat cell line (originally called JM) was established in the late 1970s from the peripheral blood of a 14 year old boy with T cell leukemia. ^[1] Different derivatives of the Jurkat cell line can now be obtained from cell culture banks^[2] that have been mutated to lack certain genes.

Examples of derivatives

• The JCaM1.6 cell line is deficient in Lck kinase activity due to the deletion of part of the lck gene (exon 7) from the Lck transcript.

• J.RT3-T3.5 cells lack the beta chain of the T cell receptor. This affects the cells in several ways. They do not express CD3 or produce the T cell receptor alpha/beta heterodimer. Since they are deficient in the TCR complex, these cells are a useful tool for transfection studies using T cell receptor alpha and beta chain genes.

• The I 9.2 and I 2.1 cell lines. The I 9.2 cell line is functionally defective for FADD and the I 2.1 cell line is functionally defective for caspase-8, both defective molecules being essential to apoptosis or programmed cell death of cells.

• The D1.1 cell line does not express CD4 molecule, an important co-receptor in the activation pathway of helper T cells.

• The J.gamma1 subline contains no detectable phospholipase C-gamma1 (PLC-γ1) protein and therefore has profound defects in T cell receptor (TCR) calcium mobilization, and nuclear factor of activated T-cells (NFAT) activation (an important transcription factor in T cells).

References

1. ^ Schneider U, Schwenk H, Bornkamm G (1977). "Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma". Int J Cancer 19 (5): 621-6. PMID 68013.
2. ^ American Type Culture Collection (ATCC)