Fungiculture

Fungiculture is the process of producing food, medicine, and other products by the cultivation of mushrooms and other fungi.

The word is commonly used to refer to the practice of cultivating fungi by Leafcutter ants, termites, ambrosia beetles, and marsh snails.

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Introduction

Mushrooms are not plants, and require different conditions for optimal growth. Plants develop through photosynthesis, a process that converts atmospheric carbon dioxide into carbohydrates, especially cellulose. While sunlight provides an energy source for plants, mushrooms derive all of their energy and growth materials from their growth medium, through biochemical decomposition processes. This does not mean that light is an unnecessary requirement, since some fungi use light as a signal for fruiting ^[1][2]. However, all the materials for growth must already be present in the growth medium. Mushrooms grow well at relative humidity levels of around 95-100%, and substrate moisture levels of 50 to 75%. ^[1]
   
Instead of seeds, mushrooms reproduce sexually during underground growth, and asexually through spores. Either of these can be contaminated with airborne microorganisms, which will interfere with mushroom growth and prevent a healthy crop.

Mycelium, or actively growing mushroom culture, is placed on growth substrate to seed or introduce mushrooms to grow on a substrate. This is also known as inoculation, spawning or adding spawn. Its main advantages are to reduce chances of contamination while giving mushrooms a firm beginning. Spores are another inoculation option, but are less developed than established mycelium. Since they are also contaminated easily, they are only manipulated in laboratory conditions with laminar flow cabinet.

Techniques

All mushroom growing techniques require the correct combination of humidity, temperature, substrate (growth medium) and inoculum (spawn or starter culture).  Wild harvests, outdoor log inoculation and indoor trays all provide these elements.

Wild harvesting

Due to its climate, the Pacific Northwest produces commercially valuable mushrooms. Valued species include ^[3][4][4]:

• American matsutake or pine mushroom (Tricholoma magnivelare)
• Chanterelles (Cantharellus formosus, subalbidus and cibarius)
• Horn of plenty (Craterellus cornucopioides)    
• Boletes (Boletus edulis and others)
• Truffles (Tuber gibbosum and Leucangium carthusiana )
• Hedgehogs / "spreading-hedgehog mushroom" (Hydnum repandum )
• Edible morel (Morchella esculenta)
• Coral tooth mushroom (Hericium abietis)
• Shaggy parasol (Lepiota rhacodes)
• Black picoa (Picoa cathusiana)     
• Cauliflower mushroom (Sparassis crispa)

Mushroom gatherers have the fewest requirements to begin business. Gatherers only need to supply funds for possible park fees, knowledge for identifying mushrooms and gathering time.

There are significant disadvantages to relying on natural mushroom production. These sales may be unregulated, placing buyers at risk for buying toxic or inedible mushrooms. By honest error, harvests may include toxic or inedible species. No controls exist to regulate the quality or frequency of harvests, since gatherers rely on favorable natural conditions and weather to produce fruiting. Conflicts may arise between competing gatherers trying to harvest from the same location.

State parks in the Pacific Northwest or elsewhere may charge fees for mushroom gathering permits ^[5][6]. Appalachia also produces edible wild mushrooms, including chanterelles and morels ^[7]. Pickers may sell directly to distributors, restaurants, or sell their harvest through roadside stands wherever a natural supply of mushrooms is plentiful.

While there may be concern that harvesting wild mushrooms may exploit or damage a natural environment, harvesting wild mushrooms is different from harvesting wild plants, fishing or hunting animals. In these last three cases, removing individuals decreases the ability of a wild population to reproduce, since fewer adults remain. Removing adults leaves fewer individuals capable of reproducing and reduces genetic diversity.

Harvesting wild mushrooms removes only fruiting bodies and their attached spores. However, the fruiting bodies (mushrooms) have likely dropped spores before harvest time. While truffles also represent the fruiting body of a larger underground network, they are an exception, since they rely on animal spore dispersion.

Additionally, reproduction and propagation can still occur by propagation of the parent mycelium. Harvesting removes none of the parent mycelium, which remains intact underground.

Additionally, reproduction and propagation can still occur by propagation of the parent mycelium. Harvesting removes none of the parent mycelium, which remains intact underground.

Outdoor logs

 
Mushrooms can be grown on logs placed outdoors in stacks or piles, as has been done for hundreds of years ^[8] . Sterilization is not performed in this method.
Since production may be unpredictable and seasonal, less than 5% of commercially sold mushrooms are produced this way [19] ^[9]

. Here, tree logs are inoculated with spawn, then allowed to grow as they would in wild conditions. Fruiting, or pinning, is triggered by seasonal changes, or by briefly soaking the logs in cool water ^[8].Shiitake and oyster mushrooms have traditionally been produced using the outdoor log technique, although controlled techniques such as indoor tray growing or artificial logs made of compressed substrate have been substituted. ^{[9] [10] [11]}

Indoor trays

Indoor growing provides the ability to tightly regulate temperature and humidity while excluding contaminants and pests. This allows consistent production, regulated by spawning cycles^[12].

Indoor tray growing is the most common commercial technique followed by containerized growing. The tray technique provides the advantages of scalability and easier harvesting. Unlike wild harvests, indoor techniques provide tight control over growing substrate composition and growing conditions. Indoor harvests are much more predictable.

"[Indoor m]ushroom farming consists of six steps, and although the divisions are somewhat arbitrary, these steps identify what is needed to form a production system. The six steps are phase I composting, phase II composting, spawning, casing, pinning, and cropping." ^[13][14]

Six phases of mushroom cultivation

Complete sterilization is not always required or performed during composting. In some cases, a pasteurization step is not included to allow some beneficial microorganisms to remain in the growth substrate ^[13] .

Specific time spans and temperatures required during stages 3-6 will vary respective to species and variety. Substrate composition and the geometry of growth substrate will also effect the ideal times and temperatures.

Pinning is the trickiest part for a mushroom grower, since a combination of CO2 concentration, temperature, light, and humidity triggers mushrooms towards fruiting ^[13][1][2]. Up until the point when rhizomorphs or mushroom "pins" appear, the mycelium is an amorphous mass spread throughout the growth substrate, unrecognizable as a mushroom.

CO2 concentration becomes elevated during the vegetative growth phase, when mycelium is sealed in a gas-resistant plastic barrier or bag which traps gases produced by the growing mycelium. To induce pinning, this barrier is opened or ruptured. CO2 concentration then decreases from about 0.08% to 0.04%, the ambient atmospheric level ^[13] .

Substrates

Mushroom production converts raw natural ingredients into the cell walls of mushroom tissue, most notably the carbohydrate chitin ^[1].

An ideal substrate will contain enough nitrogen and carbohydrate for rapid mushroom growth. Common bulk substrates include ^{[14] [12]}:

• wood chips or sawdust
• mulched hay
• strawbedded horse or poultry manure
• corncobs
• waste or recycled paper ^[15]
• coffee pulp or grounds ^{[16] [17]}
• nut and seed hulls
• cottonseed hulls
• cocoa bean hulls
• cottonseed meal
• soybean meal
• brewer’s grain
• ammonium nitrate
• urea

Mushrooms metabolize complex carbohydrates in their substrate into glucose, which is then transported through the mycelium as needed for growth and energy. While it is used as a main energy source, its concentration in the growth medium should not exceed 2%. For ideal fruiting, closer to 1% is ideal.^[1]

Pests and Diseases

Parasitic insects, bacteria and other fungi all pose risks to indoor production. The sciarid fly or phorid fly may lay eggs in growth medium, which hatch into worms and damage developing mushrooms during all growth stages. Bacterial blotch caused by Pseudomonas bacteria or patches of Trichoderma green mold also pose a risks during the fruiting stage. Pesticides and sanitizing agents are available to use against these infestations ^{[12] [18]}.Biological controls for insect sciarid and phorid flies have also been proposed ^[19].

A recent epidemic of Trichoderma green mold has significantly affected mushroom production: "From 1994-96, crop losses in Pennsylvania ranged from 30 to 100%" [4]. ^[20]

Production regions

Pennsylvania is the top-producing mushroom state in the USA, and celebrates September as "mushroom month." ^[21] The borough of Kennett Square is a historical and present leader in mushroom production. It currently leads production of Agaricus-type mushrooms, ^[22] followed by California, Florida and Michigan. ^[23]

Other mushroom-producing states:^[24]

• East: CT, DE, FL, MD, NY, PA, TN, and VT.
• Central: IL, OK, TX, and WI.
• West: CA, CO, MT, OR, UT, and WA.

Vancouver, BC also has a significant number of producers. [16] ^[25]

References