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Aldolase A




Aldolase A, fructose-bisphosphate
PDB rendering based on 4ALD.
Available structures: 1ado, 1ald, 1ewd, 1ewe, 1ewg, 1ex5, 1j4e, 1zah, 1zai, 1zaj, 1zal, 2ald, 2ot0, 2ot1, 4ald, 6ald
Identifiers
Symbol(s) ALDOA; ALDA; MGC10942; MGC17716; MGC17767
External IDs OMIM: 103850 MGI: 87994 Homologene: 68996
RNA expression pattern

More reference expression data

Orthologs
Human Mouse
Entrez 226 11674
Ensembl ENSG00000149925 ENSMUSG00000030695
Uniprot P04075 Q5FWB7
Refseq NM_000034 (mRNA)
NP_000025 (protein) 		NM_007438 (mRNA)
NP_031464 (protein)
Location Chr 16: 29.97 - 29.99 Mb Chr 7: 126.59 - 126.59 Mb
Pubmed search [1] [2]

Aldolase A is an enzyme which catalyses one of the aldol reactions: The substrate, fructose 1,6-bisphosphate (F-1,6-BP) is broken down into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP). This reaction is a part of glycolysis. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.[1]

β-D-fructose 1,6-phosphate fructose bisphosphate aldolase D-glyceraldehyde 3-phosphate dihydroxyacetone phosphate
+
fructose bisphosphate aldolase

Compound C05378 at KEGG Pathway Database. Enzyme 4.1.2.13 at KEGG Pathway Database. Compound C00111 at KEGG Pathway Database. Compound C00118 at KEGG Pathway Database.

The numbering of the carbon atoms indicates the fate of the carbons according to their position in fructose 6-phosphate.

Mechanism

In mammalian aldolase the key catylitic amino acid residues involved in the reaction are lysine and tyrosine. The tyrosine acts as an efficient hydrogen acceptor while the lysine covalently binds and stabilizes the intermediates. Many bacteria use two magnesium ions in place of the lysine.

 

References

  1. ^ Entrez Gene: ALDOA aldolase A, fructose-bisphosphate.

Further reading

  • Pfleiderer G, Thöner M, Wachsmuth ED (1976). "Histological examination of the aldolase monomer composition of cells from human kidney and hypernephroid carcinoma.". Beiträge zur Pathologie 156 (3): 266-79. PMID 766744.
  • Rehbein-Thöner M, Pfleiderer G (1977). "The changes in aldolase isoenzyme pattern during development of the human kidney and small intestine--demonstrated in organ extracts and tissue sections.". Hoppe-Seyler's Z. Physiol. Chem. 358 (2): 169-80. PMID 844801.
  • Wachsmuth ED (1976). "Differentiation of epithelial cells in human jejunum: localization and quantification of aminopeptidase, alkaline phosphatase and aldolase isozymes in tissue sections.". Histochemistry 48 (2): 101-9. PMID 955981.
  • Lee KN, Maxwell MD, Patterson MK, et al. (1992). "Identification of transglutaminase substrates in HT29 colon cancer cells: use of 5-(biotinamido)pentylamine as a transglutaminase-specific probe.". Biochim. Biophys. Acta 1136 (1): 12-6. PMID 1353685.
  • Dawson SJ, White LA (1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin.". J. Infect. 24 (3): 317-20. PMID 1602151.
  • Mukai T, Arai Y, Yatsuki H, et al. (1991). "An additional promoter functions in the human aldolase A gene, but not in rat.". Eur. J. Biochem. 195 (3): 781-7. PMID 1999195.
  • Gamblin SJ, Davies GJ, Grimes JM, et al. (1991). "Activity and specificity of human aldolases.". J. Mol. Biol. 219 (4): 573-6. PMID 2056525.
  • Vértessy BG, Orosz F, Ovádi J (1991). "Modulation of the interaction between aldolase and glycerol-phosphate dehydrogenase by fructose phosphates.". Biochim. Biophys. Acta 1078 (2): 236-42. PMID 2065091.
  • Takasaki Y, Takahashi I, Mukai T, Hori K (1990). "Human aldolase A of a hemolytic anemia patient with Asp-128----Gly substitution: characteristics of an enzyme generated in E. coli transfected with the expression plasmid pHAAD128G.". J. Biochem. 108 (2): 153-7. PMID 2229018.
  • Gamblin SJ, Cooper B, Millar JR, et al. (1990). "The crystal structure of human muscle aldolase at 3.0 A resolution.". FEBS Lett. 262 (2): 282-6. PMID 2335208.
  • Kishi H, Mukai T, Hirono A, et al. (1988). "Human aldolase A deficiency associated with a hemolytic anemia: thermolabile aldolase due to a single base mutation.". Proc. Natl. Acad. Sci. U.S.A. 84 (23): 8623-7. PMID 2825199.
  • Izzo P, Costanzo P, Lupo A, et al. (1987). "A new human species of aldolase A mRNA from fibroblasts.". Eur. J. Biochem. 164 (1): 9-13. PMID 3030757.
  • Inagaki H, Haimoto H, Hosoda S, Kato K (1988). "Aldolase C is localized in neuroendocrine cells.". Experientia 44 (9): 749-51. PMID 3046960.
  • Freemont PS, Dunbar B, Fothergill-Gilmore LA (1988). "The complete amino acid sequence of human skeletal-muscle fructose-bisphosphate aldolase.". Biochem. J. 249 (3): 779-88. PMID 3355497.
  • Izzo P, Costanzo P, Lupo A, et al. (1988). "Human aldolase A gene. Structural organization and tissue-specific expression by multiple promoters and alternate mRNA processing.". Eur. J. Biochem. 174 (4): 569-78. PMID 3391172.
  • Maire P, Gautron S, Hakim V, et al. (1988). "Characterization of three optional promoters in the 5' region of the human aldolase A gene.". J. Mol. Biol. 197 (3): 425-38. PMID 3441006.
  • Kukita A, Yoshida MC, Fukushige S, et al. (1987). "Molecular gene mapping of human aldolase A (ALDOA) gene to chromosome 16.". Hum. Genet. 76 (1): 20-6. PMID 3570299.
  • Tolan DR, Niclas J, Bruce BD, Lebo RV (1987). "Evolutionary implications of the human aldolase-A, -B, -C, and -pseudogene chromosome locations.". Am. J. Hum. Genet. 41 (5): 907-24. PMID 3674018.
  • Sakakibara M, Mukai T, Hori K (1985). "Nucleotide sequence of a cDNA clone for human aldolase: a messenger RNA in the liver.". Biochem. Biophys. Res. Commun. 131 (1): 413-20. PMID 3840020.
  • Ovádi J, Mohamed Osman IR, Batke J (1983). "Interaction of the dissociable glycerol-3-phosphate dehydrogenase and fructose-1,6-bisphosphate aldolase. Quantitative analysis by an extrinsic fluorescence probe.". Eur. J. Biochem. 133 (2): 433-7. PMID 6406231.
This metabolism related enzyme article is a stub. You can help Wikipedia by expanding it.
 v • d • e 
Glycolysis Metabolic Pathway
Glucose Hexokinase Glucose-6-phosphate Phosphoglucoisomerase Fructose 6-phosphate Phosphofructokinase Fructose 1,6-bisphosphate Fructose bisphosphate aldolase Dihydroxyacetone phosphate Glyceraldehyde 3-phosphate Triosephosphate isomerase Glyceraldehyde 3-phosphate Glyceraldehyde phosphate dehydrogenase
ATP ADP ATP ADP NAD+ + Pi NADH + H+
+ 2
NAD+ + Pi NADH + H+
1,3-Bisphosphoglycerate Phosphoglycerate kinase 3-Phosphoglycerate Phosphoglycerate mutase 2-Phosphoglycerate Enolase Phosphoenolpyruvate Pyruvate kinase Pyruvate Pyruvate dehydrogenase Acetyl-CoA
ADP ATP H2O ADP ATP CoA + NAD+ NADH + H+ + CO2
2 2 2 2 2 2
ADP ATP H2O
v • d • e
Carbon-carbon lyases (EC 4.1)
4.1.1: Carboxy-lyasesPyruvate decarboxylase -Oxaloacetate decarboxylase - Acetoacetate decarboxylase - Malonyl-CoA decarboxylase - Glutamate decarboxylase - Ornithine decarboxylase - Lysine decarboxylase - Phosphoribosylaminoimidazole carboxylase - Histidine decarboxylase - Uridine monophosphate synthetase/Orotidine 5'-phosphate decarboxylase - Aromatic L-amino acid decarboxylase - Phosphoenolpyruvate carboxylase - Pyrophosphomevalonate decarboxylase - Uroporphyrinogen III decarboxylase - RuBisCO - Phosphoenolpyruvate carboxykinase - Adenosylmethionine decarboxylase
4.1.2: Aldehyde-lyasesAldolase A/Aldolase B - 2-hydroxyphytanoyl-CoA lyase
4.1.3: Oxo-acid-lyases3-hydroxy-3-methylglutaryl-CoA lyase
v • d • e
Carbohydrate metabolism: glycolysis/gluconeogenesis enzymes
GlycolysisGlucokinase/Hexokinase/Glucose 6-phosphatase - Glucose isomerase - Phosphofructokinase 1/Fructose 1,6-bisphosphatase - Aldolase - Triosephosphate isomerase - Glyceraldehyde 3-phosphate dehydrogenase - Phosphoglycerate kinase - Phosphoglycerate mutase - Enolase - Pyruvate kinase
Gluconeogenesis onlyPyruvate carboxylase - Phosphoenolpyruvate carboxykinase - from lactate (Cori cycle): Lactate dehydrogenase - from alanine (Alanine cycle): Alanine transaminase
RegulatoryPhosphofructokinase 2/Fructose 2,6-bisphosphatase - Bisphosphoglycerate mutase
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Aldolase_A". A list of authors is available in Wikipedia.
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