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ElectrochromatographyElectrochromatography is a chemical separation technique in analytical chemistry, biochemistry and molecular biology used to resolve and separate mostly large biomolecules such as proteins. It is a combination of size exclusion chromatography (gel filtration chromatography) and gel electrophoresis. These separation mechanisms operate essentially in superposition along the length of a gel filtration column to which an axial electric field gradient has been added. The molecules are separated by size due to the gel filtration mechanism and by electrophoretic mobility due to the gel electrophoresis mechanism. Additionally there are secondary chromatographic solute retention mechanisms.[1] [2] Additional recommended knowledgeSee also
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This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Electrochromatography". A list of authors is available in Wikipedia. |
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