EcoRII

EcoRII is an restriction enzyme obtained of Escherichia coli, a Gram-negative bacteria. This enzyme recognizes asymmetric, pseudopalindromic dsDNA sequences, and cuts generating sticky ends.

Additional recommended knowledge

Daily Visual Balance Check

Weighing the right way

Don't let static charges disrupt your weighing accuracy

Cut diagram:

5' NNCCWGGNN
3' NNGGWCCNN 

5' NN  CCWGGNN
3' NNGGWCC  NN

W = A or T

References

• REBASE - The Restriction Enzyme Database
• Griffiths, J .F. A. et al. (2002): Genética. McGraw-Hill Interamericana, ISBN 84-486-0368-0.