Bismarck brown Y

  Bismarck brown Y, or Bismarck brown, Manchester brown, Phenylene brown, Basic Brown 1, or C.I. 21000, is a diazo dye. It is used in histology for staining tissues. It stains acid mucins to yellow color. It can be used with live cells. It is also used to stain cartilage in bone specimens. It is also used as one of Kasten's Shiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA.

Additional recommended knowledge

Essential Laboratory Skills Guide

Weighing the right way

Daily Sensitivity Test

It was more common in the past; today it is partially replaced by other stains.

Bismarck brown Y is a constituent of Papanicolaou stains.

It can also be used as a counterstain for Victoria blue R for staining of acid-fast microorganisms.

Its CAS number is [10114-58-6] and its SMILES structure is Nc1ccc(/N=N/c2cccc(c2)/N=N/c3ccc(N)cc3N)c(N)c1.