Biotinylated retinoids

Biotinylated retinoids are derivatives of retinol (vitamin A) carrying a biotin group for use in the isolation and purification of Retinol Binding Proteins involved in the visual cycle. The first biotinylated retinoid was synthesized in 2002 ^[1] and was used in the isolation and characterization of RPE65.^[2]

Notes and references

1. ^ Nasri Nesnas, Robert R. Rando, Koji Nakanishi “Synthesis of Biotinylated Retinoids for Cross-Linking and Isolation of Retinol Binding Proteins;” Tetrahedron 2002, 58, 6577-6584.[1]
2. ^ Wan Jin Jahng, Charles David, Nasri Nesnas, Koji Nakanishi, Robert R. Rando “A Cleavable Affinity Biotinylating Agent Reveals a Retinoid Binding Role for RPE65;” Biochemistry 2003, 42 (20), 6159-6168.[2]