Apaf-1

Apaf-1 (apoptotic protease activating factor 1) is a cytosolic protein involved in cell death or apoptosis. When Cytochrome c is released from the mitochondria, it interacts with Apaf-1 and dATP to form the apoptosome, a large oligomeric protein complex which can activate caspase 9.

Additional recommended knowledge

Essential Laboratory Skills Guide

Daily Sensitivity Test

Weighing the right way

The crystal structure of this protein was solved in April of 2005 by the laboratory of Yigong Shi[1].

The actual mechanism for this reaction is still debated though work published in April of 2006 from the laboratory of Guy Salvesen suggests that the apoptosome may induce caspase 9 dimerization and subsequent autocatalyzation [2].